CAR-T & Gene Editing Research Associate II

Crispr Therapeutics AG; San Francisco (CA); Presencial; USD 85.000 - 92.000; Jornada completa

Descripción de la vacante

CRISPR Therapeutics, Inc. in San Francisco, CA, is seeking a Research Associate II to join the CRISPR-X team developing gene editing therapies for human disease.

You will work hands-on with primary human T cells and in-vivo models to build and characterize CAR T cells generated by in-vivo gene insertion. The role requires strong immunology knowledge, flow cytometry skills, and experience with molecular biology workflows, including cloning and qPCR/ddPCR, with data kept in ELNs and shared across

Formación

• BS/MS in Immunology, Biology, Bioengineering, Genetics, or related field.
• 2–4+ years relevant research experience, or 0–2+ years with an advanced degree.
• Hands-on culture of primary human T cells including activation and expansion.
• CAR T cell engineering with transgene delivery and functional characterization.
• Strong immunology background with T cell biology knowledge.
• Excellent multicolor flow cytometry skills and data analysis.
• Knowledge of cloning, PCR, and qPCR/ddPCR.
• Highly organized with rigorous documentation and cross-functional collaboration.

Responsabilidades

• Isolate, activate, engineer, and expand primary human T cells for in-vitro studies.
• Design CAR T cell constructs and transgene delivery methods.
• Perform molecular biology workflows: cloning, plasmid and RNA prep, DNA/RNA extraction, PCR.
• Design ddPCR assays to quantify transgene integration efficiency and copy number.
• Design and execute T cell phenotyping and functional assays including cytotoxicity and cytokine release.
• Collaborate cross-functionally to support in-vivo studies and data analysis.
• Generate high-quality datasets and maintain ELNs; present data to the team.

Conocimientos

Immunology knowledge

Flow cytometry expertise

Molecular biology techniques

Analytical problem-solving

Documentation & record keeping

Team collaboration

Educación

Bachelor/Master in Immunology or related field

Herramientas

Flow cytometer

PCR/ddPCR

Cell culture equipment

Descripción del empleo

CRISPR Therapeutics, Inc. in San Francisco, CA, is seeking a Research Associate II to join the CRISPR-X team developing gene editing therapies for human disease.

You will work hands-on with primary human T cells and in-vivo models to build and characterize CAR T cells generated by in-vivo gene insertion. The role requires strong immunology knowledge, flow cytometry skills, and experience with molecular biology workflows, including cloning and qPCR/ddPCR, with data kept in ELNs and shared across