CAR-T & Gene Editing Research Associate II

Crispr Therapeutics Inc; San Francisco (CA); On-site; USD 85,000 - 92,000; Full time

Job summary

CRISPR Therapeutics, Inc. in San Francisco is seeking a Research Associate II to advance the CRISPR-X program, working at the interface of immunology and next‑generation gene editing.

You will culture and engineer primary human T cells, perform CAR T cell design, and contribute to in‑vivo studies with tLNP and other delivery modalities. Strong hands‑on cell culture, flow cytometry, and molecular biology skills are required.

Qualifications

• BS/MS in Immunology, Biology, Bioengineering, Genetics, or related field.
• 2–4+ years of relevant research experience for RA II, or 0–2+ years with advanced degree.
• Hands-on experience culturing primary human T cells: isolation, activation, expansion, cryopreservation.
• Hands-on CAR T cell engineering: transgene delivery and functional characterization.
• Strong immunology background with understanding of T cell biology, activation, and effector function.
• Expertise in multicolor flow cytometry: panel design, instrument operation, data analysis.
• Molecular biology basics: cloning, PCR, qPCR/ddPCR.
• Highly organized with rigorous records; collaborative cross-functional mindset.

Responsibilities

• Isolate, activate, engineer, and expand primary human T cells; evaluate gene insertion and CAR expression.
• Perform CAR T cell design and transgene delivery methods (nucleofection/electroporation, viral transduction, tLNP).
• Support molecular biology workflows: cloning, plasmid/RNA prep, DNA/RNA extraction, PCR.
• Design ddPCR assays to quantify transgene integration efficiency and copy number.
• Design and execute multiparameter flow cytometry panels and functional assays (cytotoxicity, proliferation, cytokine release).
• Support in-vivo studies: tissue processing, sample prep, immunophenotyping of samples.
• Generate reproducible data; maintain ELNs and present results to teams.

Skills

T cell culture

CAR T engineering

Flow cytometry

Molecular biology

Data recording

Cross-functional collaboration

Education

Immunology/Biology/Bioengineering degree

Tools

Flow cytometer

qPCR/ddPCR

Cloning

Job description

CRISPR Therapeutics, Inc. in San Francisco is seeking a Research Associate II to advance the CRISPR-X program, working at the interface of immunology and next‑generation gene editing.

You will culture and engineer primary human T cells, perform CAR T cell design, and contribute to in‑vivo studies with tLNP and other delivery modalities. Strong hands‑on cell culture, flow cytometry, and molecular biology skills are required.