Biologics Production Scientist I: Mammalian Expression

PharmaEssentia Innovation Research Center; Bedford (MA); In loco; USD 90.000 - 120.000; Tempo pieno

Descrizione del lavoro

PharmaEssentia Innovation Research Center (PIRC) seeks a Scientist I, Biologics Production to join the Biologics Platform Team. The role focuses on medium- to high-throughput mammalian cell culture using CHO and HEK systems, transient expression, purification, and analytical characterization of recombinant proteins and antigens.

You will implement scalable workflows, optimize throughput and reproducibility, work with internal teams and CROs, and contribute to project decisions through rigorous

Competenze

• Ph.D. in Biochemistry, Biophysics, Chemical Engineering, Cell Biology, Molecular Biology, or a related scientific discipline, with 0-2 years of relevant industry or academic experience; or MS in a related discipline, with 6+ years of industry experience.
• Experience producing antibodies, antigens, multispecifics, or other protein-based therapeutics.
• Demonstrated experience performing 24- to 96-well recombinant-protein expression and purification workflows in mammalian systems.
• Experience with protein purification using FPLC/ÄKTA systems, including development and troubleshooting of chromatography-based purification methods.
• Experience with fit-for-purpose characterization of recombinant proteins using techniques such as SDS‑PAGE, SEC, BLI, and/or SPR.
• Demonstrated ability to independently advance experimental work, troubleshoot technical challenges, and communicate scientific findings clearly.
• Strong organizational, documentation, and data-analysis skills, with the ability to manage multiple protein-production activities in a dynamic research environment.
• Collaborative, flexible, and team-oriented mindset, with the ability to work effectively across internal and external research partners.

Mansioni

• Execute medium- to high-throughput mammalian cell culture and recombinant protein expression workflows, including transient expression approaches.
• Develop methods and techniques to improve throughput, reproducibility, turnaround time, and production capacity in support of biologics discovery programs.
• Purify recombinant proteins using FPLC/ÄKTA-based chromatography workflows.
• Perform fit-for-purpose protein quality and characterization assessments to guide production, purification, and downstream study decisions.
• Troubleshoot expression, purification, yield, purity, stability, quality, and throughput challenges; identify and implement practical solutions.
• Collaborate with internal research teams, CROs, and external partners to coordinate protein-production activities, communicate technical requirements, and integrate results into project decisions.
• Maintain clear experimental documentation and prepare high-quality scientific reports, presentations, and data summaries.
• Contribute scientific input within project teams and stay current with emerging technologies in protein expression, purification, and biologics platform development.

Conoscenze

High-throughput mammalian cell culture

Protein expression workflows

Analytical characterization

Formazione

Ph.D. in Biochemistry / Biophysics / Chemical Engineering / Cell Biology / Molecular Biology or related

M.S. in related field with 6+ years industry experience

Strumenti

FPLC/ÄKTA systems

LC-MS

SDS-PAGE/SEC/BLI/SPR

Descrizione del lavoro

PharmaEssentia Innovation Research Center (PIRC) seeks a Scientist I, Biologics Production to join the Biologics Platform Team. The role focuses on medium- to high-throughput mammalian cell culture using CHO and HEK systems, transient expression, purification, and analytical characterization of recombinant proteins and antigens.

You will implement scalable workflows, optimize throughput and reproducibility, work with internal teams and CROs, and contribute to project decisions through rigorous