Biological Scientist - Genomics & Stem Cell Research

Guidehouse Inc.; Rockville (MD); Vor Ort; USD 65.000 - 108.000; Vollzeit

Zusammenfassung

Guidehouse Inc. in Rockville, MD is seeking a Biological Scientist to support NIH programs, designing and executing molecular, cellular, and genomic studies using genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics.

The role involves developing and applying gene expression analyses, creating genetic constructs with CRISPR/Cas, performing cell culture with primary and stem cells, and coordinating data analysis with bioinformatics teams.

Qualifikationen

• Master's degree in Biology, Molecular Biology, or a related life sciences field.
• Minimum of ONE (1) year of experience performing molecular biology techniques, including cloning, gene expression analysis, and genome engineering.
• Background supporting cell culture, stem cell biology, and cellular assay development activities.
• Practical experience with genomic technologies, including PCR, qPCR, NGS, transcriptomics, and sequencing workflows.
• Prior work analyzing experimental data and preparing technical reports and scientific documentation.
• Hands-on experience operating laboratory instrumentation and maintaining laboratory quality standards.
• Knowledge of laboratory safety procedures, quality control practices, and research documentation requirements.
• Ability to work effectively in a multidisciplinary research environment.
• Must be able to obtain and maintain a Federal or DoD 'public trust'; candidates must receive approved adjudication prior to onboarding with Guidehouse.

Aufgaben

• Design, execute, and optimize laboratory studies utilizing genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics technologies.
• Design, execute, and optimize molecular biology experiments using gene expression analysis and functional genomics; design and generate genetic constructs through molecular cloning, plasmid engineering, CRISPR/Cas genome editing, and viral vector preparation.
• Isolate, quantify, and assess DNA, RNA, and proteins using manual and automated workflows; prepare and process samples, including library preparation and quality control, for PCR, qPCR, NGS, transcriptomics, and single-cell sequencing to generate genomic and transcriptomic data. Coordinate downstream analyses with bioinformatics teams.
• Coordinate cell line quality control, including mycoplasma testing, karyotyping, STR/DNA fingerprinting, contamination monitoring, and genetically modified cell line validation; manage cell banking activities, including cryopreservation, recovery, authentication, distribution, shipment, receipt, and documentation.
• Perform and optimize mammalian cell culture experiments with primary cells, stem cells, immortalized cell lines, and differentiated populations; differentiate stem cells into specialized cell types using established and novel protocols, automated culture systems, and high-throughput platforms.
• Perform protein characterization and cellular analyses using Western blotting, immunocytochemistry, and immunofluorescence staining; prepare and analyze tissue specimens using cryostat sectioning, microscopy, and histological techniques. Perform fluorescence and confocal imaging and quantitative image analysis.
• Develop, validate, and implement fluorescence-, bioluminescence-, and reporter-based high-throughput screening assays; perform cellular functional assays using flow cytometers, Seahorse metabolic flux analyzers, and multi-electrode arrays.
• Collect, analyze, and interpret experimental data; prepare technical reports, manuscripts, scientific publications, and presentations, and support grant applications. Participate in weekly laboratory meetings to present project updates, findings, and research progress.
• Maintain inventories of reagents, antibodies, plasmids, cell lines, consumables, and experimental resources; operate, calibrate, troubleshoot, and maintain laboratory instrumentation and analytical equipment, including fluorescence and confocal imaging systems. Monitor laboratory operations and infrastructure and coordinate maintenance and instrument services.
• Prepare and maintain protocols, SOPs, electronic laboratory notebooks, regulatory and technical documentation, and quality records to ensure compliance and reproducibility; train laboratory personnel on equipment operation, laboratory procedures, and best practices.
• Provide multidisciplinary teams with expertise in molecular biology, genome engineering, stem cell biology, assay development, and translational research to advance program objectives and collaborative research initiatives.

Kenntnisse

Molecular biology techniques

Genome engineering

Cell culture

Genomic technologies

Data analysis

Technical documentation

Multidisciplinary collaboration

Ausbildung

Master's degree in Biology, Molecular Biology, or a related life sciences field

Tools

PCR

qPCR

NGS

Flow cytometry

Confocal imaging

Seahorse

Jobbeschreibung

Guidehouse Inc. in Rockville, MD is seeking a Biological Scientist to support NIH programs, designing and executing molecular, cellular, and genomic studies using genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics.

The role involves developing and applying gene expression analyses, creating genetic constructs with CRISPR/Cas, performing cell culture with primary and stem cells, and coordinating data analysis with bioinformatics teams.