Molecular & Stem Cell Scientist (Genomics)

Guidehouse; Rockville (MD); On-site; USD 65,000 - 108,000; Full time

Benefits offered by this job

Medical, Rx, Dental & Vision Insurance

Personal and Family Sick Time

Company Paid Holidays

Parental Leave

401(k) Retirement Plan

Group Term Life and Travel Assistance

Voluntary Life and AD&D Insurance

Health Savings Account, Health Care &

Job summary

Guidehouse in Rockville, MD is seeking a Biological Scientist to support NIH programs involving molecular biology, genomics, and translational research. You will design, execute, and optimize laboratory studies using genome engineering, stem cell biology, sequencing, imaging, and functional genomics.

Masters in Biology or related field with hands-on experience in cloning, CRISPR, cell culture, and genomic workflows is required; the role includes grant support and collaboration across

Qualifications

• Master's degree in Biology, Molecular Biology, or a related life sciences field.
• Minimum of ONE (1) year of experience performing molecular biology techniques, including cloning, gene expression analysis, and genome engineering.
• Background supporting cell culture, stem cell biology, and cellular assay development activities.
• Practical experience with genomic technologies, including PCR, qPCR, NGS, transcriptomics, and sequencing workflows.
• Prior work analyzing experimental data and preparing technical reports and scientific documentation.
• Hands‑on experience operating laboratory instrumentation and maintaining laboratory quality standards.
• Knowledge of laboratory safety procedures, quality control practices, and research documentation requirements.
• Ability to work effectively in a multidisciplinary research environment.
• Must be able to obtain and maintain a Federal or DoD “public trust”.

Responsibilities

• Design, execute, and optimize laboratory studies using genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics.
• Design and generate genetic constructs through molecular cloning, plasmid engineering, CRISPR/Cas genome editing, and viral vector preparation.
• Isolate, quantify, and assess DNA, RNA, and proteins; prepare libraries for PCR, qPCR, NGS, transcriptomics, and single-cell sequencing.
• Coordinate downstream analyses with bioinformatics teams.
• Coordinate cell line quality control, mycoplasma testing, karyotyping, STR/DNA fingerprinting, contamination monitoring, and validation; manage cell banking activities.
• Perform and optimize mammalian cell culture, differentiation, and automated culture systems; maintain cell lines.
• Perform protein characterization and cellular analyses using Western blotting, immunocytochemistry, and immunofluorescence; prepare tissue sections and histology.
• Develop, validate and implement high-throughput screening assays; perform cellular assays using flow cytometers, Seahorse analyzers, and multi-electrode arrays.
• Collect, analyze, and interpret data; prepare technical reports, manuscripts and grant applications; present findings at meetings.

Skills

Genome engineering

Stem cell biology

High-throughput screening

Sequencing

Imaging

Functional genomics

Gene expression analysis

Molecular cloning

CRISPR/Cas

Viral vector preparation

Cell culture

Data analysis

Grant applications

Education

Master's degree in Biology, Molecular Biology, or related life sciences

Tools

Seahorse metabolic flux analyzers

Flow cytometers

NGS instruments

Confocal microscope

PCR/qPCR

Job description

Guidehouse in Rockville, MD is seeking a Biological Scientist to support NIH programs involving molecular biology, genomics, and translational research. You will design, execute, and optimize laboratory studies using genome engineering, stem cell biology, sequencing, imaging, and functional genomics.

Masters in Biology or related field with hands-on experience in cloning, CRISPR, cell culture, and genomic workflows is required; the role includes grant support and collaboration across