Automation Scientist - LVV/AAV Vector Production

R&D Partners; South San Francisco (CA); On-site; USD 59,000 - 65,000; Full time

Benefits offered by this job

Medical insurance – comprehensive

401k plan

Employee Assistance Program

Long-term disability

Weekly payroll

Online timecard approval

Job summary

R&D Partners in South San Francisco, CA seeks a Scientist to accelerate LVV and AAV generation platforms, focusing on automating high-throughput workflows and improving production throughput for rapid screening and pipeline studies.

This 6-month contract involves implementing automated liquid handling, mini-scale bioreactors, and DOE-driven process development to optimize upstream/downstream operations and analytics for quality vectors.

Qualifications

• MS/PhD degree in Cell Biology, Molecular Biology, Virology, Biochemistry, Bioengineering, Genetics, or a related discipline.
• 3+ years of laboratory research or industrial experience.
• Proficient in mammalian cell culture and general molecular biology techniques such as transient plasmid transfection, PCR, qPCR, ddPCR, dPCR and Western blot.
• Require strong data analysis and presentation skills.
• Previous experience with ex vivo CAR T engineering using lentivirus is a must.
• Working experiences with lentivirus or AAV production in adherent or suspension cells, process development, statistical DOE modeling and automated liquid handling are highly desirable.
• Experience with flow cytometer operations, electronic notebook and BSL2 facility management are also strong plus.
• Must be self-motivated, detail-oriented, analytical, highly organized, and have excellent communication and interpersonal skills.

Responsibilities

• Integrate automation and high-throughput LVV and AAV workflows.
• Implement and program automated liquid handling systems for high-throughput transfection, transduction, sample dilution and cell seeding.
• Incorporate automated shaker arrays or micro-scale bioreactor platforms to run parallel expression runs.
• Troubleshoot liquid-class parameters, pipetting precision, and plasticware compatibility to minimize variation.
• Develop and optimize suspension-based LVV upstream production platform.
• Design, execute, and characterize robust upstream processes from seed train expansion through harvest.
• Apply Design of Experiments to explore multivariate interactions including transfection conditions and plasmid ratios to boost packaging efficiency.
• Develop and establish downstream LVV process.
• Evaluate and optimize low-shear depth filtration and microfiltration protocols for large-scale virus production.
• Develop and optimize ultrafiltration/diafiltration and chromatographic purification methodologies.
• Conduct analytics for LVV and AAV QC.
• Perform characterization of viral vectors to interpret quality attributes.
• Integrate QC in process development for rapid identification of improvement factors.
• Data, Inventory and Protocol Management: analyze multivariate datasets to facilitate project progression.
• Maintain virus inventory and registry using digital informatics platforms for data integrity.
• Author SOPs, technical reports and tech transfer protocols.
• Present data in group and department meetings.

Skills

Mammalian cell culture

Molecular biology techniques

Data analysis

DOE/experimental design

Team collaboration

Education

MS/PhD degree in Cell Biology or related

Tools

Integra Assist Plus

Bravo

Ambr 15

Ambr 250

Flow cytometer

Electronic notebook

Job description

R&D Partners in South San Francisco, CA seeks a Scientist to accelerate LVV and AAV generation platforms, focusing on automating high-throughput workflows and improving production throughput for rapid screening and pipeline studies.

This 6-month contract involves implementing automated liquid handling, mini-scale bioreactors, and DOE-driven process development to optimize upstream/downstream operations and analytics for quality vectors.