Scientist, Autoimmunity, SIgN

A*STAR RESEARCH ENTITIES; Singapore; On-site; SGD 60,000 - 90,000; Full time

Job summary

The Lab of Immune Regulation, Resolution & Tolerance at Singapore Immunology Network (SIgN), part of A*STAR, invites a motivated postdoctoral scientist to advance our understanding of immune tolerance in autoimmunity, focusing on SLE and lupus nephritis. The successful candidate will lead experiments and collaborate across disciplines.

Responsibilities include in vivo mouse models, high-parameter flow cytometry, in vitro assays, handling human samples under ethical approvals, and analyzing

Qualifications

• PhD in Biomedical Sciences/Engineering with Immunology specialization.
• Strong understanding of immunology and autoimmunity.
• Peer-reviewed publications relevant to career stage.
• Experience designing experiments with high technical rigor.
• Familiarity with cell culture, primary immune cell isolation and flow cytometry.
• Familiarity with next generation sequencing including spatial transcriptomics and TCRseq+scRNAseq.
• Excellent problem solving and communication abilities.

Responsibilities

• Investigate dynamic immune composition and cross-talk during disease progression from SLE to lupus nephritis.
• Perform in vivo mouse experiments and handle mouse breeding and procedures.
• Design, optimize and analyze flow cytometry experiments across mouse and human tissues.
• Conduct in vitro assays including FACS and immune cell culture.
• Handle human clinical samples under regulatory and ethical approvals.
• Collaborate to set up and analyze sequencing datasets (TCR-seq, spatial transcriptomics).
• Analyze data using FlowJo, R and Python; contribute to team mentoring and collaboration.

Skills

In vivo mouse model experiments

High-parameter flow cytometry

FACS

Cell culture of primary immune cells

Bioinformatics (R, Python)

Education

PhD in Biomedical Sciences/Engineering (Immunology specialization)

Tools

FlowJo

R

Python

TCR sequencing

Spatial transcriptomics

Job description

The Lab of Immune Regulation, Resolution & Tolerance at Singapore Immunology Network (SIgN) is seeking a motivated and interdisciplinary postdoctoral scientist to join our team in the field of autoimmunity.

The successful candidate will lead and contribute to advancing our understanding of immune tolerance is affected in autoimmunity, namely in Systemic Lupus Erythematosus (SLE) and lupus nephritis (LN). In particular, the candidate will explore myeloid and lymphocyte crosstalk during disease progression, and investigate regulatory T cell deficits in SLE utilizing pre-clinical mouse models, with the opportunity to expand their findings to human samples.

Job Requirements

Key Responsibilities:

Investigate the dynamic changes in immune composition and cross-talk during disease progression from SLE to lupus nephritis

• Conduct in vivo mouse model experiments, including mouse breeding, handling, surgical procedures, end-point sample harvesting, ensuring compliance with regulatory and ethical requirements.
• Design, perform, optimise and analyse high parameter flow cytometry experiments across both mouse and human tissues.
• Conduct in vitro wet lab experiments, such as fluorescence activated cell sorting (FACS), cell culture of primary immune cells, immunofluorescence staining, among others.
• Handle and manage human clinical samples, ensuring compliance with regulatory and ethical requirements.
• Collaborate with others to set-up and analyse large sequencing datasets including TCR-sequencing and spatial transcriptomics.
• Analyse data using a combination of standard software including Flowjo (flow cytometry) and bioinformatics tools (R, Python).

Team contribution, mentoring and collaboration

• Provide mentorship and guidance to students and junior researchers in the lab
• Engage and work closely with multidisciplinary team of collaborators across Singapore
• Work closely with the PI to align research priorities and meet milestones

Job Requirements:

• Ph.D. in Biomedical Sciences/Engineering, with an Immunology specialization strongly preferred.
• Strong understanding of immunology, with a key focus on autoimmunity.
• Strong track record of peer-reviewed publications appropriate to the current career stage
• Experience in designing and executing experiments with a high degree of technical rigour. Familiarity with cell culture, primary immune cell isolation and flow cytometry is preferred.
• Familiarity with next generation sequencing, especially for spatial transcriptomics and TCRseq+scRNAseq is a plus.
• Excellent problem solving and critical thinking skills with the ability to work independently and independently within a team environment.
• Strong written and oral communication skills, with experience in scientific publication and presentations

Why join us:

• Be part of a young and dynamic new team advancing research into immune tolerance and resolution
• Gain experience working at the intersection of wet and dry lab with access to state-of-the-art facilities and technologies
• Perform rigorous science with strong translational capabilities