Scientist, Neutrophil BIology

A*STAR - Agency for Science, Technology and Research; Singapore; On-site; SGD 60,000 - 80,000; Full time

Benefits offered by this job

State-of-the-art facilities

Mentorship opportunities

Dynamic research environment

Job summary

A*STAR - Agency for Science, Technology and Research in Singapore is seeking a motivated postdoctoral scientist to advance research in neutrophil biology. The successful candidate will lead projects using cutting-edge sequencing techniques and conduct experiments in mouse models of inflammation and cancer.

This role offers the opportunity to mentor students and collaborate with a multidisciplinary team. Applicants should hold a Ph.D. in Biomedical Sciences/Engineering and possess a strong background in immunology. Join us in our dynamic new team!

Qualifications

• Ph.D. in Biomedical Sciences/Engineering with Immunology specialization preferred.
• Strong understanding of immunology and cancer biology.
• Experience with cell culture and flow cytometry is preferred.

Responsibilities

• Investigate spatial and temporal neutrophil reprogramming in inflammation.
• Conduct in vivo mouse model experiments and in vitro wet lab experiments.
• Mentor students and junior researchers in the lab.

Skills

Immunology

Neutrophil biology

Single-cell RNA sequencing (scRNAseq)

Flow cytometry

Data analysis (R, Python)

Education

Ph.D. in Biomedical Sciences/Engineering

Tools

Flowjo

CRISPR

Job description

Overview

The Lab of Immune Regulation, Resolution & Tolerance at Singapore Immunology Network (SIgN) is seeking a motivated and interdisciplinary postdoctoral scientist to join our team in the field of neutrophil biology. The successful candidate will lead projects that contribute to advancing our understanding of how neutrophil heterogeneity impacts inflammation resolution and tolerance. They will have access to cutting‑edge sequencing and spatial transcriptomics techniques to dissect the role of neutrophil subsets in mouse models of inflammation and cancer, and subsequently apply their findings to human samples.

Key Responsibilities

• Investigate how neutrophil reprogramming is spatially and temporally directed by the local environment in inflammation and cancer
• Design, analyse and interpret single-cell RNAsequencing (scRNAseq) and spatial transcriptomics datasets, with the specific conservation of neutrophils.
• Conduct in vivo mouse model experiments, including mouse handling, surgical procedures, end‑point sample harvesting, ensuring compliance with regulatory and ethical requirements.
• Conduct in vitro wet lab experiments, including cell culture of both cell lines and primary neutrophils, immunofluorescence staining, among others.
• Design, perform and optimise a wide array of experiments using techniques such as flow cytometry, fluorescence activated cell sorting, CRISPR-based editing.
• Handle and manage human clinical samples, ensuring compliance with regulatory and ethical requirements.
• Analyse data using a combination of standard software including Flowjo (flow cytometry) and bioinformatics tools (R, Python).

Team Contribution, Mentoring and Collaboration

• Provide mentorship and guidance to students and junior researchers in the lab
• Engage and work closely with multidisciplinary team of collaborators across Singapore
• Work closely with the PI (Melissa Ng), to align research priorities and meet milestones

Job Requirements

• Ph.D. in Biomedical Sciences/Engineering, with an Immunology specialization strongly preferred.
• Strong understanding of immunology and cancer biology, with a focus on neutrophil biology.
• Strong track record of peer-reviewed publications appropriate to the current career stage
• Experience in designing and executing experiments with a high degree of technical rigour. Familiarity with cell culture, primary immune cell isolation and flow cytometry is preferred.
• Familiarity with next generation sequencing, especially for spatial transcriptomics and scRNAseq is a plus.
• Excellent problem-solving and critical thinking skills with the ability to work independently and collaboratively within a team environment.
• Strong written and oral communication skills, with experience in scientific publication and presentations

Why Join Us

• Be part of a young and dynamic new team advancing research into immune tolerance and resolution
• Gain experience working at the intersection of wet and dry lab with access to state-of-the-art facilities and technologies
• Perform rigorous science with strong translational capabilities