Cardiac Gene Therapy Research Technician

Werkenbijumcutrecht; Utrecht; On-site; EUR 35,000 - 57,000; Full time

Benefits offered by this job

Year-end bonus

Holiday allowance

ABP pension

Public transport reimbursement

Bike/benefits options

Job summary

The Experimental Cardiology group at UMC Utrecht is seeking a research technician to produce, purify, and characterize engineered extracellular vesicles for cardiac gene therapy applications and evaluate their therapeutic potential in preclinical models.

You will combine mammalian cell culture, EV manufacturing and purification, and molecular assays to support the development of innovative gene therapies for cardiovascular diseases.

Qualifications

• Bachelor's or Master's degree in Biotechnology, Life Sciences, Molecular Biology, or a related field.
• Hands-on experience with mammalian cell culture and aseptic techniques.
• Experience with molecular biology techniques including Amplicon sequencing, PCR and DNA/RNA isolation.
• Ability to independently perform experiments and troubleshoot technical challenges.
• Strong organizational skills, attention to detail, and a proactive attitude.
• Good communication in Dutch and English.

Responsibilities

• Maintain and scale mammalian cell cultures for EV production.
• Perform large-scale EV production and purification.
• Characterize EVs for size, cargo, concentration, purity and protein content.
• Conduct molecular assays (DNA, RNA, protein) and sequencing-based analyses.
• Plan, execute, troubleshoot experiments, and document results.

Skills

Lab experience

Independent working

Organizational skills

Communication skills

Dutch & English

Education

Bachelor's or Master's in Biotechnology/Life Sciences

Tools

Mammalian cell culture

Aseptic techniques

Amplicon sequencing

PCR

DNA/RNA isolation

Western blotting

Flow cytometry

Fluorescence-based assays

Job description

The Experimental Cardiology group at UMC Utrecht is seeking a research technician to produce, purify, and characterize engineered extracellular vesicles for cardiac gene therapy applications and evaluate their therapeutic potential in preclinical models.

You will combine mammalian cell culture, EV manufacturing and purification, and molecular assays to support the development of innovative gene therapies for cardiovascular diseases.