CRISPR Genomics Research Assistant: Bone&Cartilage (Hybrid)

University of Manchester; Manchester; Hybrid; GBP 32,000 - 42,000; Full time

Benefits offered by this job

Pension scheme

Health & wellbeing services

Annual leave + holidays

Christmas closure

Discounts

Job summary

The University of Manchester is seeking a motivated Research Assistant to join the Rice Lab in the Division of Musculoskeletal and Dermatological Sciences. This two-year post will contribute to a programme of functional genomics research examining how genetic variation influences gene regulation in osteoarthritis and skeletal traits.

The successful candidate will use CRISPR-Cas9 genome editing in human cell models, culture primary human cells, and characterise regulatory-element deletions during

Qualifications

• Knowledge of mammalian cell culture, aseptic technique and core molecular biology principles.
• Ability to plan and prioritise multi-stage experimental work and deliver reproducible results.
• Ability to analyse, interpret and clearly present experimental data in written and oral formats.
• Experience of primary human cell culture and/or processing human tissue samples.
• Experience of mesenchymal stromal/stem cell culture or osteogenic and/or chondrogenic differentiation.

Responsibilities

• Delivering a defined programme of experimental research under the direction of Dr Sarah Rice, planning and prioritising day-to-day experimental activity and regularly discussing progress and priorities.
• Culturing and maintaining established human cell lines, mesenchymal stromal cells and primary human cells using high-quality aseptic technique.
• Generating and validating targeted regulatory-element deletions and other experimental cell models using CRISPR-Cas9 genome editing and related molecular approaches.

Skills

Mammalian cell culture

Aseptic technique

Molecular biology

Experimental design

Data analysis

Tools

CRISPR-Cas9 genome editing

Job description

The University of Manchester is seeking a motivated Research Assistant to join the Rice Lab in the Division of Musculoskeletal and Dermatological Sciences. This two-year post will contribute to a programme of functional genomics research examining how genetic variation influences gene regulation in osteoarthritis and skeletal traits.

The successful candidate will use CRISPR-Cas9 genome editing in human cell models, culture primary human cells, and characterise regulatory-element deletions during