Functional Skeletal Genomics Research Assistant

The University of Manchester; Manchester; Hybrid; GBP 29,000 - 36,000; Full time

Benefits offered by this job

Pension scheme

Health and wellbeing services

Annual leave

Christmas closure

Retail discounts

Job summary

The University of Manchester is seeking a motivated Research Assistant to join the Rice Lab in the Division of Musculoskeletal and Dermatological Sciences. This two-year post will contribute to a programme of functional genomics research investigating how genetic variation influences gene regulation and cellular function in osteoarthritis and other skeletal traits.

The post will involve CRISPR-Cas9 genome editing in human cell models and culture of primary human cells.

Qualifications

• Knowledge of mammalian cell culture and aseptic technique.
• Experience with genome editing methods (e.g., CRISPR-Cas9).
• Ability to plan multi-stage experimental work and deliver reproducible results.
• Ability to analyse, interpret and clearly present experimental data.

Responsibilities

• Delivering a defined programme of experimental research under the direction of Dr Sarah Rice, planning and prioritising day-to-day experimental activity and regularly discussing progress and priorities.
• Culturing and maintaining established human cell lines, mesenchymal stromal cells and primary human cells using high-quality aseptic technique.
• Generating and validating targeted regulatory-element deletions and other experimental cell models using CRISPR-Cas9 genome editing and related molecular approaches.

Skills

Mammalian cell culture

Aseptic technique

Molecular biology

Data analysis

Tools

CRISPR-Cas9 genome editing

Job description

The University of Manchester is seeking a motivated Research Assistant to join the Rice Lab in the Division of Musculoskeletal and Dermatological Sciences. This two-year post will contribute to a programme of functional genomics research investigating how genetic variation influences gene regulation and cellular function in osteoarthritis and other skeletal traits.

The post will involve CRISPR-Cas9 genome editing in human cell models and culture of primary human cells.