Photonic microscopy engineer

France - BioImaging; Illkirch-Graffenstaden; Sur place; EUR 42 000 - 56 000; Plein temps

Résumé du poste

BioImaging and Pathologies Laboratory in France is seeking a candidate to implement and validate TRAST spectroscopy, a fluorescence technique to probe dark-state dynamics, and integrate it into the PIQ-QuESt imaging platform for users in academia and industry.

The role covers instrumental development, alignment of a laser-based setup, validation with chromophore Cy5, and building a real-time data analysis and software workflow for TRAST signals.

Qualifications

• Hands-on photonic hardware experience (lasers, modulators, detectors).
• Solid understanding of optical alignment and beam propagation.
• Proficiency in MATLAB, Python, LabVIEW or Arduino.
• Familiarity with fluorescence microscopy data processing.
• Basic knowledge of (bio)molecular systems and photophysics.
• English proficiency at B2 level or higher.

Responsabilités

• Instrumental development: assemble and align a laser-based setup.
• Instrument validation: demonstrate performance with a benchmark chromophore.
• Data analysis pipeline: develop software tools for real-time TRAST data.

Connaissances

Hands-on photonic hardware

Optical alignment

MATLAB

Python

LabVIEW

Arduino

Outils

Lasers

Modulators

Detectors

sCMOS camera

Description du poste

Organization: BioImaging and Pathologies Laboratory

The successful candidate will implement and validate Transient-State (TRAST) spectroscopy, a fluorescence technique designed to probe fluorophore dark-state dynamics. The method will then be integrated into the PIQ-QuESt imaging platform and made available to academic and industrial users.

Main missions

• Instrumental development – Assembly and align a complete laser based set-up (laser sources, AOM, beam delivery optics, and sCMOS camera detector),
• Instrument Validation – Demonstrate performance with a benchmark chromophore that populates dark states (e.g., the photo isomerisation dynamics of the pentamethine cyanine Cy5),
• Data analysis pipeline – Implement, test and document software tools for real time acquisition and quantitative analysis of TRAST data.

Profile

Required knowledges

• Hands-on experience with photonic spectroscopy hardware (lasers,
  modulators, detectors),
• Solid understanding of optical alignment and beam propagation principles.
• Proficiency in at least one programming environment: MATLAB, Python,
  LabVIEW or Arduino,
• Familiarity with fluorescence microscopy data processing,
• Basic knowledge of (bio)molecular systems and their photophysics,
• English proficiency at B2 level (or higher).

Desired skills and personal qualities

• Precision and rigor in experimental work; strong troubleshooting mindset,
• Ability to write clear user protocols and to contribute to scientific
  publications,
• Good visual and oral presentation skills,
• Proven team spirit and ability to collaborate effectively within a
  multidisciplinary group.