Externally Funded Doctoral Student (m/f/d) – Epigenetic Regulation of Pancreatic Cancer Cell Pl[...]

TN Germany; Stuttgart; Vor Ort; EUR 40.000 - 70.000; Vollzeit

Zusammenfassung

An established industry player is seeking an externally funded doctoral student to explore the epigenetic regulation of pancreatic cancer cell plasticity. This exciting position involves utilizing cutting-edge sequencing-based molecular biology techniques to investigate gene regulatory mechanisms and their impact on tumor cell identity and treatment response. The role promises a vibrant international research environment, where you will collaborate with a dedicated team, gain expertise in modern epigenetic methods, and contribute to groundbreaking cancer research. If you're passionate about molecular biology and eager to push the boundaries of science, this opportunity is perfect for you.

Leistungen

Internationale Forschungsumgebung

Moderne Infrastruktur

Unterstützendes Team

Qualifikationen

• Abschluss eines Masterstudiums in einem verwandten Bereich.
• Erfahrung in Zellkultur und molekularbiologischen Techniken.

Aufgaben

• Durchführung molekularbiologischer Analysen zur Untersuchung epigenetischer Mechanismen.
• Dokumentation von Arbeitsabläufen und Datenanalysen.

Kenntnisse

Molekularbiologie

Bioinformatik

Zellkultur

Transfektionen

Real-Time PCR

Western Blot

Englischkenntnisse

Ausbildung

Master of Science in Biologie

Doktorandenstipendium

Tools

10X Genomics Technologie

Akoya PhenoImager

Jobbeschreibung

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Externally Funded Doctoral Student (m/f/d) – Epigenetic Regulation of Pancreatic Cancer Cell Plasticity (Johnsen group), Stuttgart

Location:

Stuttgart, Germany

Job Category:

Other

EU work permit required:

Yes

Job Reference:

4aae443512f1

Job Views:

1

Posted:

02.05.2025

Expiry Date:

16.06.2025

Job Description:

• Utilize modern sequencing-based molecular biology approaches (RNA-seq, ChIP-seq, HiChIP, Hi-C, PRO-seq, etc.) to test hypotheses and examine epigenetic and gene regulatory mechanisms controlling pancreatic tumor cell identity and therapy response.
• Bioinformatic analysis and interpretation of data followed by hypothesis testing and generation.
• Perform multiplex immunofluorescence staining to confirm gene regulatory mechanisms in primary tumor tissue using multispectral imaging with an Akoya PhenoImager.
• Prepare histological samples (FFPE tumor material) for single cell RNA-sequencing using 10X Genomics technology.
• Conduct advanced cell culture experiments to examine the effects of drug treatment in various translational model systems.
• Participate in internal or external research collaborations.
• Document work procedures, data acquisition, and analyses.

Qualifications

• Completed a Master of Science in a related field (biology, molecular biology, biotechnology, or similar).
• Must have secured or be able to secure external funding for their position (e.g., doctoral fellowship from DAAD, China Scholarship Council, or similar).
• Experience with cell culture, transfections, etc.
• Extensive molecular biology experience (real-time PCR, Western blot, etc.).
• Strong interest in learning and gaining proficiency in bioinformatic analyses.
• Strong work ethic and high level of self-motivation.
• Independent, goal-oriented, and structured working style with the ability to work in a team.
• Confident written and spoken English skills.

Additional Information

We offer you:

• An exciting and international research environment.
• Excellent experience and expertise in modern epigenetic methods.
• Outstanding bioinformatic expertise.
• Extensive modern infrastructure for all methods related to the project.
• Working in a committed team, characterized by mutual appreciation and support.
• Proven track record of successfully supervising more than 20 doctoral students.
• Long track record in gene regulation and cancer research.

This position is contingent upon the applicant having attained or attaining an externally funded fellowship for the duration of their project.

Contact:

Prof. Dr. Steven A. Johnsen
Scientific Director
E-Mail: