Stem Cell Scientist

Ovelle; Boston (MA); On-site; USD 140,000 - 190,000; Full time

Benefits offered by this job

Paid holidays

PTO

Parental leave

Job summary

Ovelle in Boston is seeking a Stem Cell Scientist to optimize and execute iPSC differentiation protocols, critical to advancing in vitro oogenesis. You will lead efforts to identify regulators of ovarian cell fate and develop 3D ovarian organoids, collaborating with bioinformatics to analyze RNA sequencing data.

The role offers the chance to apply cutting-edge stem cell biology to a field with global health impact, with opportunities to advance this groundbreaking fertility technology.

Qualifications

• PhD or track record of innovation with focus on stem cell biology and differentiation protocols.
• Experience with human pluripotent stem cells and directed differentiation protocols.
• Proficiency in genetic engineering techniques including plasmid design and cloning, CRISPR/Cas9-mediated genome editing, and gene delivery systems.
• Experience with flow cytometry, FACS and immunofluorescence microscopy including data analysis.
• Familiarity with sequencing technologies, particularly single-cell RNA-seq, and basic bioinformatics skills.
• Proven ability to execute complex, multi-step cell culture protocols and 3D organoid systems.

Responsibilities

• Lead optimization and execution of iPSC differentiation protocols.
• Design transcription factor screening experiments to identify key regulators of ovarian cell fate.
• Develop and characterize 3D ovarian organoids by co-culturing iPSC-derived granulosa-like cells with meiotic oogonia.
• Collaborate with bioinformatics to interpret bulk and single-cell RNA sequencing data.

Skills

iPSC differentiation

Pluripotent stem cells

Genetic engineering

CRISPR/Cas9

Geno delivery systems

FACS

Immunofluorescence microscopy

RNA-seq (single-cell & bulk)

Bioinformatics basics

Education

PhD in Biology/Biochemistry or related field

Tools

CRISPR/Cas9 genome editing

Lentiviral/transposon delivery systems

Flow cytometry (FACS)

Immunofluorescence microscopy

Single-cell RNA-seq

Bulk RNA-seq data analysis

Job description

About the Company

Ovelle is revolutionizing fertility treatment through human in vitro oogenesis—producing eggs from somatic cells. We have developed technology to create the cell types of the ovary from human induced pluripotent stem cells (iPSCs) by manipulating expression of transcription factors and RNA‑binding proteins. Notably, we are the first company to produce meiotic oocyte‑like cells from human iPSCs. This is an important step towards in vitro oogenesis because activating meiosis has been a key challenge in the field. We are incorporating these meiotic oocyte‑like cells into ovarian organoids to create synthetic ovaries capable of producing eggs. By innovating egg production, we aim to make fertility treatments more accessible and affordable. If we are successful, it will mark a paradigm shift in treatments for infertility, and offer a new path to parenthood for millions of people around the world. Come help us build the exciting future we all want to live in.

About the Specific Role

We seek a Stem Cell Scientist to optimize and execute iPSC differentiation protocols. In this role you will lead this effort, which is critical to the success of human in vitro oogenesis. This position offers a unique opportunity to apply your skills in a field with immense potential to impact global health and happiness.

How You Will Contribute

• Lead the optimization and execution of iPSC differentiation protocols.
• Design and implement transcription factor screening experiments to identify key regulators of ovarian cell fate.
• Develop and characterize 3D ovarian organoids (ovaroids) by co‑culturing iPSC‑derived granulosa‑like cells with meiotic oogonia.
• Collaborate with our bioinformatics team to interpret bulk and single‑cell RNA sequencing data, contributing to the understanding of ovarian cell development and function.

Qualifications

• PhD in Biology, Biochemistry, or a closely related field; or a track record of innovation.
• Experience working with human pluripotent stem cells and directed differentiation protocols.
• Proficiency in genetic engineering techniques, including plasmid design and cloning, CRISPR/Cas9‑mediated genome editing, lentiviral/transposon‑based gene delivery systems, and pooled library screens.
• Experience with flow cytometry, fluorescence‑activated cell sorting (FACS), and immunofluorescence microscopy, including experimental design and data analysis.
• Familiarity with sequencing technologies, particularly single‑cell RNA‑seq, and basic bioinformatics skills for data analysis.
• Proven track record of successful execution of complex, multi‑step cell culture protocols and 3D organoid systems.

Our Benefits

• 12.5 paid holidays
• 20 days PTO
• Up to 12 weeks paid leave for new parents who are primary caregivers
• Perk MBTA pass program
• Medical, Dental, and Vision
• Matching 401(k) plan after first year
• Tuition reimbursement for work‑related continuing education