Stem Cell Culture & Lab Operations Specialist

iORGANBIO; Durham (NC); On-site; USD 41,000 - 50,000; Full time

Benefits offered by this job

Equity

Job summary

iOrganBio is hiring a Cell Culture Technician for an in-person, full-time role in Durham, NC. The position involves maintaining healthy human cell lines, executing differentiation workflows, and supporting high-throughput experiments under a regulated, data-driven environment.

Equity will be provided and mentorship from world-class scientists is available. The role emphasizes aseptic technique, detailed documentation, and collaboration with automation and sequencing teams to advance stem cell

Qualifications

• Demonstrated experience with aseptic technique and human cell culture.
• Proficiency with standard lab techniques and equipment including pipetting, sterile handling, biosafety cabinets, centrifugation, and basic molecular assays.

Responsibilities

• Culture and maintain human pluripotent and differentiated cells under sterile conditions.
• Perform routine feeding, passaging, thawing, cryopreservation, and cell banking.
• Support development and optimization of directed differentiation workflows.
• Conduct QC checks including morphology assessments, viability, and confluency tracking.
• Perform basic molecular biology workflows including PCR, electrophoresis, and nucleic acid quantification.
• Assist with preparation of sequencing libraries, including single-cell RNA-seq workflows.
• Execute molecular biology sample QC using instruments.
• Support high-throughput library preparation workflows, including operation of automated liquid handling systems.
• Prepare, label, and ship samples for external sequencing while maintaining chain-of-custody and data integrity.
• Prepare media, reagents, consumables, and maintain sterile workspaces.
• Operate key lab equipment including BSCs, incubators, centrifuges, microscopes.
• Uphold laboratory organization and contribute to general lab maintenance.
• Accurately document experimental activities in ELNs and LIMS.
• Follow SOPs and contribute to updates and continuous improvement of procedures.
• Assist with sample handling, labeling, and tracking to ensure data integrity.
• Work with automation and sequencing teams to support integrated experimentation.
• Communicate clearly with scientists and engineers regarding observations and needs.

Skills

Aseptic technique

Cell culture

Documentation

Flexibility

Job description

iOrganBio is hiring a Cell Culture Technician for an in-person, full-time role in Durham, NC. The position involves maintaining healthy human cell lines, executing differentiation workflows, and supporting high-throughput experiments under a regulated, data-driven environment.

Equity will be provided and mentorship from world-class scientists is available. The role emphasizes aseptic technique, detailed documentation, and collaboration with automation and sequencing teams to advance stem cell