Staff Research Associate - Pediatrics

UCSF Health, AG; San Francisco (CA); On-site; USD 65,000 - 90,000; Full time

Job summary

The Huang Lab at UCSF is seeking a Staff Research Associate to conduct leukemia research using mice and cell lines. You will perform cell culture, biochemical assays, DNA/RNA work, and generate/transduce hematopoietic cells, with careful record-keeping of mouse colonies.

Required BA/BS in a related science. Preferred skills include mouse breeding and molecular biology techniques such as PCR and Western blotting.

Qualifications

• BA/BS in a related science; or an equivalent combination of education and experience.
• Experience with cell culture and molecular biology techniques is favorable.

Responsibilities

• Conduct leukemia research using mouse models and cell lines.
• Perform biochemical assays (e.g., Western blotting, immunoprecipitation, qPCR).
• Maintain accurate records and manage mouse colonies including genotyping.

Skills

Cell culture

Molecular biology

PCR/qPCR

Flow cytometry

Education

BA/BS in a related science

Tools

Western blotting

DNA/RNA extraction

Lentiviral/retroviral vector generation

Job description

Job Description

The Huang Lab at UCSF ( https://huanglabucsf.com/ ) investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include: (1) to determine the biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to identify genes and biochemical pathways that are regulated differentially in leukemia versus normal cells; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipient mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic agents, radiation, and new experimental treatments to mice.

Job Description

The Huang Lab at UCSF ( https://huanglabucsf.com/ ) investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include: (1) to determine the biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to identify genes and biochemical pathways that are regulated differentially in leukemia versus normal cells; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipient mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic agents, radiation, and new experimental treatments to mice.

This Staff Research Associate (SRA) position is for an individual who will conduct leukemia research using mouse models and cell lines. SRA duties will include: cell/tissue culture, biochemical assays (western blotting, immunoprecipitation, qPCR), DNA and RNA extraction and manipulation, PCR and agarose gel electrophoresis, generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells, flow cytometry, maintenance of accurate and complete records, organization and maintenance of mouse colonies (including computerized record-keeping, breeding, monitoring and genotyping), and experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues).

Qualifications

Required Qualifications:

• BA/BS in a related science; or an equivalent combination of education and experience

Preferred Qualifications

• Expertise in breeding and maintaining laboratory mice
• Molecular biology skills including DNA/RNA extraction and PCR amplification
• Tissue culture skills including passaging cell lines
• Biochemistry skills including Western blotting