Staff Research Associate - Neurology

UCSF Health, AG; San Francisco (CA); On-site; USD 65,000 - 95,000; Full time

Job summary

The Kriegstein lab at UCSF is seeking a motivated Staff Research Associate to contribute to cutting-edge functional genomics research using CRISPR libraries and single-cell technologies in human primary cells. The SRA will prepare lentiviral vectors, handle human tissues, perform infections, conduct FACS, and prepare 10x Genomics single-cell libraries, working under the PI in a collaborative setting.

Strong organizational and communication skills, attention to detail, and a BA/BS in a related

Qualifications

• BA/BS degree in a related scientific field.
• Foundational understanding of basic neuroscience concepts and standard laboratory procedures.
• Strong organizational skills with the ability to manage multiple tasks and prioritize effectively.
• Excellent written and verbal communication skills.
• Meticulous attention to detail and a commitment to accurate, thorough record keeping.

Responsibilities

• CRISPR/Lentiviral Work: Prepare lentiviral vectors for CRISPR library applications; perform lentiviral infection of cultured cells.
• Handling of Human Primary Tissues: Perform handling of human primary tissues, including tissue dissociation and cell culture of primary cells under appropriate conditions; perform fluorescence‑activated cell sorting (FACS).
• Library Preparation: Prepare single‑cell sequencing libraries using the 10x Genomics platform.
• Maintain stocks, reagents, laboratory equipment, perform other duties as assigned, keep notes about experiments updated.

Skills

Strong organizational skills

Excellent written and verbal沟通 skills

Education

BA/BS in related field

Degree in Neurobiology (preferred)

Tools

Lentiviral vectors

FACS (Flow Cytometry)

Single-cell library prep (10x Genomics)

Job description

JOB SUMMARY

The Kriegstein lab is seeking a highly motivated, enthusiastic, and organized Staff Research Associate (SRA) to join our team as a technician contributing to cutting‑edge functional genomics research. This position exists to support high‑impact studies that leverage CRISPR library screening and single‑cell technologies to investigate gene function in human primary cells.

The SRA will prepare lentiviral vectors for CRISPR library applications, handle human primary tissues (including tissue dissociation and cell culture of primary cells under appropriate conditions), perform lentiviral infection of cultured cells, carry out fluorescence‑activated cell sorting (FACS), and prepare single‑cell sequencing libraries using the 10x Genomics platform. The SRA will be expected to perform independently under the supervision of the PI, while gaining hands‑on experience with state‑of‑the‑art technology in a highly collaborative research environment.

DUTIES & ESSENTIAL JOB FUNCTIONS

• CRISPR/Lentiviral Work: Prepare lentiviral vectors for CRISPR library applications; perform lentiviral infection of cultured cells.
• Handling of Human Primary Tissues: Perform handling of human primary tissues, including tissue dissociation and cell culture of primary cells under appropriate conditions; perform fluorescence‑activated cell sorting (FACS).
• Library Preparation: Prepare single‑cell sequencing libraries using the 10x Genomics platform.
• Maintain stocks, reagents, laboratory equipment, perform other duties as assigned, keep notes about experiments updated.

Required Qualifications:

• BA/BS degree in a related scientific field
• Foundational understanding of basic neuroscience concepts and standard laboratory procedures
• Strong organizational skills with the ability to manage multiple tasks and prioritize effectively
• Excellent written and verbal communication skills
• Meticulous attention to detail and a commitment to accurate, thorough record keeping

Preferred Qualifications:

• Degree in Neurobiology