Kampmann Lab – Junior/Assistant Specialist

University of California, San Francisco; San Francisco (CA); On-site; USD 55,000 - 70,700; Full time

Job summary

University of California, San Francisco in San Francisco, CA seeks a Kampmann Lab Junior/Assistant Specialist to develop CRISPR/Cas9-based functional genomics approaches and understand disease mechanisms. The role involves team collaboration, problem solving, and potential independent projects with data analysis.

Responsibilities include in vivo mouse work, tissue processing, sequencing prep, and collaboration on CRISPR screening platforms, with a two-year commitment preferred (one-year minimum).

Qualifications

• Education options for Junior or Assistant Specialist as listed in the posting.
• Academic credentials or years of research experience as described.
• Includes coursework and training in biology, neuroscience or related fields.

Responsibilities

• Perform experiments with murine models, primary neurons, and mammalian cells.
• Conduct in vivo work, tissue collection, immunostaining, imaging and sequencing prep.
• Analyze datasets and support CRISPR-based projects.
• Assist collaborative projects using lab mouse in vivo screening platforms.
• Maintain mouse colony tasks and related administrative duties.

Skills

Organized

Attention to detail

Communication

Written English

Education

Baccalaureate degree in Biology/Neuroscience or related life sciences

Master’s degree in Biology/Neuroscience or related life sciences

Tools

MS Office

Job description

Kampmann Lab – Junior/Assistant Specialist

The Kampmann lab develops and uses innovative technologies to understand cellular and molecular mechanisms of human diseases and to discover new therapeutic strategies. A major focus of our research is on neurodegenerative diseases such as Alzheimer’s and Parkinson’s Disease. To this end, we have established a CRISPR-based functional genomics platform for genetic screens in both human iPSC-based and murine models of disease (Tian et al., 2019, Neuron; Ramani et al., 2023, bioRxiv). We are an interdisciplinary group of postdocs, graduate students and staff research associates. We are proud of our lab culture of intellectual curiosity, collaboration, support, and fun! Read more about our lab at kampmannlab.ucsf.edu.

Position Description

In this position, you will be working as a member of a research team developing innovative, CRISPR/Cas9-based functional genomics approaches to understand disease mechanisms and identify potential therapeutic strategies. You must be willing to work in a team, be organized, enjoy problem solving, and have strong communication skills. As you become familiar with our methodology, there will be opportunities to work independently on research projects. Based on your interest and expertise, you will also contribute to bioinformatics analysis of data as well.

Responsibilities

• Conduct experiments using murine models of disease, primary neuronal cultures, and mammalian cell culture under the guidance of a graduate student or postdoc.
• Perform in vivo experiments such as delivering viral vectors into mice, collecting brain tissue, sectioning and immunostaining tissues, confocal imaging, flow cytometry, and preparing samples for next-generation sequencing.
• Analyze the resulting datasets.
• Support collaborative projects that require use of the lab’s CRISPR-based mouse in vivo screening platforms.
• Spend approximately 30% of the time on mouse colony maintenance tasks including setting up breeding cages, weaning, tail/ear clipping, genotyping, responding to mouse health concerns, updating the mouse inventory database, and other administrative tasks related to the mouse facility and IACUC protocols.

Two-year commitment is preferred; the candidate must be able to make a one-year commitment.

Qualifications

• Education and Training Qualifications:
  • Junior Specialist – Baccalaureate degree in Biology, Molecular Biology, Neuroscience, or related life or medical sciences, or at least four years of research experience.
  • Assistant Specialist – Master’s degree in Biology, Molecular Biology, Neuroscience, or related life or medical sciences, or a Baccalaureate degree with at least three years of research experience.
• Technical Qualifications:
  • Working knowledge of MS Office (Word, Excel, PowerPoint).
  • Highly organized, attention to detail, demonstrated record-keeping abilities.
  • Excellent communication skills; good written English and ability to write reports.
• Preferred Qualifications:
  • Experience with mouse handling, including weaning and ear punching.
  • Experience with mammalian cell culture and primary neuronal culture.
  • Experience with sectioning and immunostaining of mouse brain tissues.
  • Experience in molecular biology, such as PCR, molecular cloning.
  • Experience in biochemistry, such as Western blotting.
  • Working knowledge of flow cytometry and microscopy.
  • Bioinformatics experience, such as analysis of imaging or next-generation sequencing data.

Document Requirements

• Curriculum Vitae – CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).
• Cover Letter.
• Statement of Research (Optional).
• Statement of Teaching (Optional).

Reference Requirements

• 3 required (contact information only).

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

UCSF is committed to welcoming and serving all people, honoring the dignity of every individual without preference or prejudice, in support of its public mission and in alignment with our PRIDE values and Principles of Community.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

• “Misconduct” means any violation of the policies or laws governing conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment or discrimination, as defined by the employer.
• UC Sexual Violence and Sexual Harassment Policy.
• UC Anti-Discrimination Policy.
• APM - 035: Affimative Action and Nondiscrimination in Employment.

San Francisco, CA

Salary: $55,000 - $70,700.

Please apply online at https://aprecruit.ucsf.edu/JPF05956 with a CV, cover letter, and three references contacts.