Senior Scientist, Single-Cell Assay Development

Themis Bios; Pleasanton (CA); On-site; USD 150,000 - 210,000; Full time

Job summary

Themis Bios in Pleasanton, CA is seeking a Senior Scientist for Applications Development to own the biology behind our single-cell RNA-seq platform. You will grow cells, develop robust sample prep, and design perturbation studies that translate into scalable workflows customers can run.

This hands-on role spans cell culture, assay development, data interpretation with computational colleagues, and cross-functional collaboration to turn experimental results into product-ready protocols.

Qualifications

• PhD in cell biology, stem cell biology, molecular biology, bioengineering, or a related field - or an MS with equivalent hands-on industry experience.
• Hands-on experience with mammalian cell culture and 3D/organoid systems.
• Experience preparing single-cell samples and scRNA-seq workflows.
• Experience designing controlled experiments with proper controls and QC metrics.
• Comfort working with scRNA-seq data and collaborating with computational colleagues.

Responsibilities

• Establish and maintain 2D and 3D culture systems, including iPSC-derived cell types and organoids, with documented quality.
• Develop and run sample prep for single-cell workflows from cells, organoids, and tissue, optimized for compatibility with the platform.
• Design, execute, and analyze perturbation experiments using drugs across dose and time with single-cell RNA-seq readouts.
• Turn working experiments into robust, transferable protocols with defined controls and QC metrics.
• Partner with computational colleagues to analyze scRNA-seq results and connect readouts to biology and experimental design.
• Collaborate with platform, chemistry, and product teams to translate results into reproducible workflows.

Skills

Mammalian cell culture

Single-cell RNA-seq

Experimental design

Data interpretation

Cross-functional collaboration

Education

PhD in cell biology or related field

Tools

Python

R

Seurat/Scanpy

Job description

Themis Bios in Pleasanton, CA is seeking a Senior Scientist for Applications Development to own the biology behind our single-cell RNA-seq platform. You will grow cells, develop robust sample prep, and design perturbation studies that translate into scalable workflows customers can run.

This hands-on role spans cell culture, assay development, data interpretation with computational colleagues, and cross-functional collaboration to turn experimental results into product-ready protocols.