Senior Scientist, Protein Purification & Enzymology

PacBio; Menlo Park (CA); Presencial; USD 129.000 - 193.000; Tempo integral

Resumo da vaga

PacBio is seeking a Senior Scientist to serve as the protein purification expert within Biochemistry and Reagents R&D. You will develop expression, purification, and characterization strategies for nucleic acid–modifying enzymes and their homologs, and build scalable purification pipelines feeding PacBio’s library preparation and sequencing chemistry.

The ideal candidate has deep hands‑on purification and mechanistic enzymology experience, strong collaboration across a multidisciplinary team,

Qualificações

• PhD in Biochemistry, Molecular Biology, Biophysics, Chemistry, Bioengineering, or related field, with 2–5 years post‑PhD industry experience.
• Hands‑on protein expression and purification of recombinant proteins/complexes, including challenging targets.
• Strong experience with FPLC-based chromatography (affinity, ion‑exchange, size‑exclusion) and method development.
• Proficiency in designing quantitative biochemical assays (kinetics, binding) and substrate design.
• Experience with high‑throughput purification on lab automation platforms (24‑ to 96‑plex).
• Track record of delivering high‑quality reagents across multiple projects.

Responsabilidades

• Build and optimize purification pipelines for nucleic acid‑modifying enzymes across research scales.
• Serve as SME on protein expression and purification; advise on constructs and purification strategy.
• Develop strategies for challenging targets; compare methods to maximize yield and purity.
• Lead high‑throughput expression/purification campaigns on lab automation platforms.
• Perform targeted purifications on ÄKTA/FPLC systems using affinity, ion‑exchange, and size‑exclusion.
• Design quantitative biochemical assays for new enzyme classes and their substrates.
• Establish QC for purified proteins (purity, concentration, activity, nuclease testing).
• Collaborate to advance enzymes from expression to evaluation in library prep workflows.
• Maintain protocols and inventory; mentor Scientists and Research Associates.
• Communicate results in meetings and reports; contribute to cross‑functional discussions.

Conhecimentos

Protein purification

Enzymology

FPLC

High-throughput purification

Protein expression

Assay development

QC methods

Team collaboration

Communication skills

Cloning/molecular biology

NGS library prep knowledge

Formação

PhD in Biochemistry/related field

Ferramentas

FPLC systems

KingFisher

Tecan

Opentrons

Hamilton

Capillary electrophoresis

DLS

SDS-PAGE

Mass spectrometry

Descrição da vaga

PacBio is seeking a Senior Scientist to serve as the protein purification expert within Biochemistry and Reagents R&D. You will develop expression, purification, and characterization strategies for nucleic acid–modifying enzymes and their homologs, and build scalable purification pipelines feeding PacBio’s library preparation and sequencing chemistry.

The ideal candidate has deep hands‑on purification and mechanistic enzymology experience, strong collaboration across a multidisciplinary team,