Biochemistry R&D Scientist I - HiFi Library Prep & Purification

1000 Pacific Biosciences of California, Inc.; Menlo Park (CA); Sur place; USD 97 000 - 168 000; Plein temps

Résumé du poste

PacBio is seeking a Scientist I or II to join the Biochemistry and Reagents R&D group. The role involves protein purification, HiFi sequencing library preparation, and targeted enrichment workflows across multiple programs.

You will learn quickly, collaborate across teams, and contribute to high-quality data generation and documentation. Ideal candidates have hands-on experience in molecular biology/biochemistry, experience with protein expression/purification, and familiarity with sequencing

Qualifications

• PhD in Biochemistry, Molecular Biology, Biophysics, Chemistry, Bioengineering, or related field.
• Scientist I: recent PhD (0–2 years) including potential postdoc.
• Scientist II: 2+ years postdoc or industry experience.
• Hands-on bench skills in molecular biology/biochemistry with multi-step protocols.
• Experience with recombinant protein expression and purification.
• Experience with NGS library preparation or similar workflows.
• Proficiency in nucleic acid and protein QC methods.
• Ability to learn new techniques quickly and manage multiple experiments.

Responsabilités

• Execute protein expression and purification protocols (FPLC-based and plate-based) with the purification lead.
• Prepare HiFi sequencing libraries and run sequencing chemistry experiments on PacBio platforms.
• Execute targeted enrichment workflows following PacBio protocols, including guide RNA designs.
• Learn new protocols quickly and move between projects as priorities change.
• Perform QC on nucleic acids and proteins and evaluate results against metrics.
• Analyze and present data in team meetings and reports.
• Maintain electronic lab notebooks and contribute to protocol improvements.
• Collaborate with Biochemistry & Reagents R&D, Bioinformatics, and Product Development.

Connaissances

Molecular biology

Biochemistry

NGS library prep

Protein purification

Recombinant protein expression

QC methods

Data analysis (Python/R)

Scientific collaboration

Effective communication

Formation

PhD in Biochemistry or related field

Postdoc experience (optional for Scientist I)

Relevant post-PhD industry experience

Outils

FPLC

HiFi sequencing platforms

Qubit

Bioanalyzer

Femto Pulse

SDS-PAGE

Description du poste

PacBio is seeking a Scientist I or II to join the Biochemistry and Reagents R&D group. The role involves protein purification, HiFi sequencing library preparation, and targeted enrichment workflows across multiple programs.

You will learn quickly, collaborate across teams, and contribute to high-quality data generation and documentation. Ideal candidates have hands-on experience in molecular biology/biochemistry, experience with protein expression/purification, and familiarity with sequencing