Senior Scientist, Bioanalysis & Biomarkers

Judo Bio; Cambridge (MA); On-site; USD 140,000 - 200,000; Full time

Benefits offered by this job

Equity

Health benefits

Retirement plan

Job summary

Judo Bio seeks a Senior Scientist to lead bioanalytical assay development and biomarker strategy in support of siRNA discovery and nonclinical programs. You will develop LC-MS/MS methods for oligonucleotides and small molecules, while overseeing CRO partners to deliver GLP-adjacent data for IND-enabling studies.

You will collaborate with DMPK, Pharmacology, Toxicology, and Regulatory to ensure regulatory-ready data packages for FIH filings, with leadership responsibilities and a focus on assay

Qualifications

• Ph.D. in analytical/bioanalytical chemistry or related field (or MS with hands-on industry experience).
• 5+ years in bioanalytical assay development using LC-MS/MS across oligonucleotide and small-molecule matrices.
• Proficiency with nanoLC-MS/MS, HRMS, and diverse chromatographic methods.
• Experience managing or overseeing CRO-generated data for nonclinical/IND programs.
• Knowledge of PK/PD bioanalysis and biomarker assay development for pharmacodynamics.
• Understanding regulatory expectations for nonclinical data supporting IND/FIH submissions.

Responsibilities

• Design, develop, and qualify LC-MS/MS and chromatographic assays for siRNA and small-molecule quantitation in various matrices.
• Lead bench work including method development, sample prep, extraction, and instrument operation.
• Develop targeted proteomics or biomarker assays to support PD readouts and PK/PD relationships.
• Manage bioanalytical CROs: transfer methods, review data, monitor timelines, troubleshoot.
• Contribute to regulatory documents (IND Module 2.4/2.6, study reports).
• Collaborate with DMPK, Toxicology, and Pharmacology to plan assay strategies for GLP toxicology and FIH studies.
• Maintain instrumentation and ensure data integrity; set QC standards across internal and CRO sites.
• Support build-out of internal bioanalytical capabilities and SOPs.

Skills

Bioanalytical leadership

Regulatory awareness

Data interpretation

Scientific writing

Cross-functional collaboration

Education

Ph.D. in analytical/bioanalytical chemistry or related

Tools

Chromeleon

XCalibur

TraceFinder

Skyline

GraphPad Prism

Mascot/Proteome Discoverer

Job description

Senior Scientist, Bioanalysis & Biomarkers

Nonclinical Development | Cambridge, MA | Full-Time | Title Commensurate with Experience

About Judo

Judo Bio is pioneering oligonucleotide medicines delivered to the kidney, opening the way for new RNA medicines for systemic and renal diseases. With our STRIKE (Selectively Targeting RNA Into KidnEy) platform, we use a proprietary approach that harnesses a natural, endogenous mechanism to target oligonucleotide medicines to the kidney.

Our initial drug programs are ligand-siRNA conjugate drugs designed for receptor-mediated uptake by specific kidney cell types, resulting in gene silencing of disease-modifying target genes for systemic diseases.

Enabled by a network of high caliber experts and internal talent, we are thoughtfully building a team of passionate, innovative, and energetic scientific champions to discover and develop therapeutics that have the potential to change the lives of patients and families.

Role Summary

Judo Bio is seeking a Senior Scientist to lead bioanalytical assay development and biomarker strategy in support of our siRNA discovery and nonclinical development programs. This role is equal parts bench scientist and program-level strategist: you will personally develop and run LC-MS/MS and chromatographic methods for oligonucleotide and small-molecule quantitation, while also managing external CRO relationships that generate GLP-adjacent and GLP bioanalytical data supporting IND-enabling studies. You will partner closely with DMPK, Pharmacology, Toxicology, and Regulatory to ensure bioanalytical and biomarker data packages are scientifically rigorous and regulatory-ready for first-in-human (FIH) filings.

Title commensurate with experience.

Key Responsibilities

• Design, develop, optimize, and qualify LC-MS/MS and chromatographic (HPLC/UPLC/FPLC/IEC) assays for quantitation of siRNA oligonucleotides, metabolites/fragments, and small-molecule conjugation partners or linker components in plasma, tissue, and other biological matrices.
• Lead hands-on bench work including method development, sample preparation, extraction, and instrument operation/troubleshooting on mass spectrometry and chromatography platforms.
• Develop and implement targeted proteomics or other biomarker assays to support pharmacodynamic (PD) readouts and strengthen PK/PD and exposure-response relationships across nonclinical programs.
• Serve as primary technical point of contact and day-to-day manager for bioanalytical CROs, including method transfer, protocol review, data review/QC, timeline management, and troubleshooting to ensure high-quality, regulatory-ready data packages.
• Author and contribute to bioanalytical and biomarker sections of nonclinical regulatory documents (e.g., IND Module 2.4/2.6, study reports, briefing documents) in collaboration with Regulatory Affairs and nonclinical leadership.
• Partner with DMPK, Toxicology, and Pharmacology teams to design bioanalytical and biomarker strategies for GLP toxicology studies and FIH-enabling packages, including matrix selection, assay validation strategy, and sample logistics planning.
• Independently analyze, interpret, and present complex analytical data to inform program-level nonclinical decision-making, candidate selection, and study design.
• Maintain instrumentation (mass spectrometers, HPLC/UPLC systems, fraction collectors) to ensure operational reliability and data integrity; establish and enforce performance-qualification standards internally and at CRO sites.
• Contribute to the build-out of internal bioanalytical capabilities and SOPs as Judo scales its nonclinical development organization.

Required Qualifications

• Ph.D. in analytical/bioanalytical chemistry, biochemistry, pharmaceutical sciences, or a related discipline (or M.S. with equivalent hands‑on industry experience).
• 5+ years of hands‑on bioanalytical experience developing and qualifying LC‑MS/MS assays, with demonstrated expertise across both oligonucleotide (e.g., siRNA, ASO, or other modified nucleic acid) and small‑molecule matrices.
• Deep hands‑on proficiency with nanoLC‑MS/MS, HRMS, and chromatographic separations (RP‑HPLC, UPLC, FPLC, IEC) including method optimization and system suitability.
• Direct experience managing or closely overseeing CRO-generated bioanalytical data, including protocol/report review and troubleshooting, in support of nonclinical or IND‑enabling studies.
• Working knowledge of PK/PD bioanalysis and biomarker assay development (e.g., targeted or bottom‑up proteomics) to support pharmacodynamic and mechanistic endpoints.
• Familiarity with regulatory expectations for nonclinical bioanalytical data supporting IND submissions and FIH study design (e.g., contributing to Module 2.4/2.6 content).
• Strong data analysis, interpretation, and technical writing skills; ability to communicate complex analytical findings clearly to cross‑functional and leadership audiences.

Preferred Qualifications

• Prior experience at a biotech developing RNAi, ASO, or other oligonucleotide-based therapeutics.
• Experience with oligonucleotide purification and characterization of chemically modified nucleic acid constructs.
• Experience contributing to GLP or GLP‑adjacent bioanalytical method validation.
• Track record of peer‑reviewed publications and/or mentorship of junior scientists.
• Proficiency with Chromeleon, XCalibur, TraceFinder, Skyline, GraphPad Prism, and/or Mascot/Proteome Discoverer.

What Judo Offers

• The opportunity to build core bioanalytical and biomarker capabilities from the ground up at a well‑funded, mission‑driven siRNA company.
• Direct, high‑visibility collaboration with nonclinical, translational, and regulatory leadership shaping first-in-human strategy.
• Competitive compensation, equity, and benefits package.

Judo Bio recognizes the value of diversity in our workforce. We are committed to equal opportunity. We consider all qualified employment applicants without regard to race, religion, color, gender, age, national origin, sexual orientation, gender identity, partnership status, protected veteran status, disability, or any other status protected by federal, state, or local law. Individuals who hold legal work authorization applicable to employment at Judo Bio in the United States will be considered without regard to citizenship/alienage.