Senior Research Technician

Dana-Farber Cancer Institute; Boston, Northern (MA, KY); Hybrid; USD 52,000 - 57,000; Full time

Job summary

The Dana-Farber Cancer Institute's TPD Proteomics Core in the Fischer Lab seeks a Senior Research Technician to apply mass spectrometry-based methods to profile cellular proteomes under stimuli. You will work with technicians, scientists, postdocs, and students on projects centered on targeted protein degradation as a novel therapeutic approach.

Located in Boston, you will contribute to proteomics workflows, QC, data analysis, and protocol improvements while maintaining rigorous lab standards

Qualifications

• Bachelor’s degree in biology, chemistry, or related field required.
• 1–4 years in mass spectrometry or molecular biology lab (academic or industry).
• Experience operating Thermo and/or Bruker mass spectrometers required.
• Experience with mammalian cell culture and proteomics sample prep preferred.
• Experience in MS data analysis and quantitative methods.
• Knowledge of statistics and R programming preferred.
• Familiarity with Proteome Discoverer, DIANN, and Spectronaut

Responsibilities

• Perform cell culture and drug treatments as part of the core team.
• Prepare proteomics samples including lysis, digestion, cleanup, and labeling.
• Run LC-MS samples on Orbitrap/timsTOF following SOPs and QC checks.
• Maintain lab notebooks and sample metadata accurately.
• Collaborate with core users to ensure timely sample processing.
• Help implement new protocols and train junior staff.
• Maintain lab supplies and liaise with purchasing/vendors.

Skills

Mass spectrometry

Cell culture

Data analysis

R programming

Education

Bachelor's degree in biology, chemistry, or related field

Tools

Thermo mass spectrometers

Bruker mass spectrometers

Proteome Discoverer

DIANN

Spectronaut

S-Trap

SP3

TMT

Job description

The Targeted Protein Degradation (TPD) Proteomics Core within the Fischer Lab at Dana-Farber Cancer Institute serves as a resource for the Cancer Biology Department, aiding investigators in answering key research questions using mass spectrometry-based proteomics.

We employ quantitative chemoproteomic and interactomic approaches to identify drug targets and are continuing to expand our repertoire to meet the needs of our core users. Our core is equipped with extensive mass spectrometry equipment including timsTOF HT, timsTOF Ultra2 and Orbitrap Eclipse tribrid mass spectrometers.

The TPD Core seeks a highly motivated individual to join the team as a Senior Research Technician, who will employ mass spectrometry-based technologies to assess the cellular proteome in response to external stimuli. This individual will be an important member of our core team and will work with technicians, scientists, postdocs and students on a variety of different research projects focused on targeted protein degradation as a novel therapeutic modality. While we consider various mass spectrometry related backgrounds, a strong interest and understanding of mass spectrometry-based proteomics is a must and strong computational skills are highly desirable.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers,

The TPD Core seeks a highly motivated individual to join the team as a Senior Research Technician, who will employ mass spectrometry-based technologies to assess the cellular proteome in response to external stimuli. This individual will be an important member of our core team and will work with technicians, scientists, postdocs and students on a variety of different research projects focused on targeted protein degradation as a novel therapeutic modality. While we consider various mass spectrometry related backgrounds, a strong interest and understanding of mass spectrometry-based proteomics is a must and strong computational skills are highly desirable.

Responsibilities:

• Responsible for cell culture and performing drug treatments
• Perform proteomics sample preparation including cell lysis, digestion, peptide cleanup (e.g., S-Trap, SP3), and labeling (e.g., TMT)
• Run samples on LC-MS systems (e.g., Orbitrap, timsTOF) following standard protocols.
• Support routine QC checks and instrument maintenance under staff supervision
• Maintain accurate and well-organized lab notebooks and sample metadata
• Collaborate with core users to ensure timely sample processing and communication
• Assist in the implementation of new protocols and improvements to existing workflows
• Help train junior staff and contribute to a clean and safe laboratory environment
• Maintains and orders laboratory supplies
• Communicates with purchasing and may be responsible for negotiating with vendors

Qualifications:

• Bachelor's degree in biology, chemistry, or related field.
• 1-4 years of experience in a mass spectrometry or molecular biology lab (academic or industry).
• Experience operating Thermo and/or Bruker mass spectrometers is required
• Experience with mammalian cell culture and proteomics sample preparation is preferred
• Experience in mass spectrometry data analysis and quantitative methods
• Knowledge of statistical and computational methods and R programming is preferred
• Knowledge of proteomics data analysis software, including Proteome Discoverer, DIANN, spectronaut
• Strong attention to detail and ability to follow SOPs.

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusiveenvironment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries and when it comes to hiring the most dedicated and compassionateprofessionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

EEO Poster

Pay Transparency Statement

The hiring range is based on market pay structures, with individual salaries determined by factors such as business needs, market conditions, internal equity, and based on the candidate’s relevant experience, skills and qualifications.

For union positions, the pay range is determined by the Collective Bargaining Agreement (CBA).

$52,400.00 - $57,200.00