Senior Research Associate, In Vitro Biology

Atalanta Therapeutics, Inc.; Boston (MA); On-site; USD 120,000 - 170,000; Full time

Job summary

Atalanta Therapeutics, Inc. seeks a highly motivated individual to join the In Vitro Biology team for preclinical development of RNAi-based therapeutics. The role involves designing and executing assays to quantify oligonucleotides and assess mRNA/protein knockdown and biomarker efficacy.

The position emphasizes building robust cell-based assays, using cloning, stable cell lines, culture and expression analysis, with independent data interpretation and meticulous record keeping.

Qualifications

• Strong background in assay development, optimization and validation in mammalian cell culture.
• Experience with oligonucleotide quantification and PD biomarker identification is preferred.

Responsibilities

• Design and implement assays for quantitation of oligonucleotides, mRNAs and proteins from cells and tissues.
• Develop and standardize cell-based assays using molecular cloning, stable cell line generation, cell culture and expression analysis.
• Independent analysis and interpretation of data, careful documentation of experiments and presentation of results.

Skills

Assay development

Mammalian cell culture

Molecular cloning

RNAseq

Data interpretation

ELISA

Stable cell lines

Communication

Oligonucleotide quant

Education

MS/MA Biochemistry

BS/BA Biology

Tools

SDS-PAGE

Immunoblotting

Job description

Atalanta is seeking a highly motivated individual to join our In Vitro Biology team to contribute to the preclinical development of RNAi-based therapeutics. Theselected individual is expected to design and execute assays for thequantification of therapeutic oligonucleotides, to assess theknockdown of target mRNAs and proteins and to assess additional molecular biomarkers of therapeutic efficacy .

· Thedesign and implementation of rigorous assays for the quantitation of oligonucleotides, mRNAs and proteins from cells and tissues.

· Developingand standardizing cell-based assays, using skills in molecular cloning, stablecell line generation, cell culture and expression analysis

· Independentanalysis and interpretation of data, careful documentation of experiments andpresentation
of results within the company

Essential Functions:

• A strong background in assay development, optimizationand validation in mammalian cell culture
• Technical expertise in cell-based bioassays,SDS-PAGE/immunoblotting and ELISA/multiplex assays
• Extensive experience in molecular biology,including molecular cloning, creation of stable cell lines for targetevaluation, mRNA expression analysis, RNAseq and DNA sequence analysis tools
• Experience with oligonucleotide quantification,pharmacodynamic biomarker identification, and neurobiology are preferred
• Ability to work and think independently whilecontributing as a part of a highly matrixed team
• Excellent communication skills includinginterpersonal communication, presentations to groups, written reports anddetailed record keeping

Education

• M.S./M.A. in biochemistry or relevant biologicalscience with an additional 5+ years of experience, or
• B.S./B.A. in a relevant biological science with anadditional 8+ years of experience

Atalanta is committed to equalemployment opportunity and non-discrimination for all employees and qualifiedapplicants without regard to a person’s race, color, gender, age, religion,national origin, ancestry, disability, veteran status, genetic information,sexual orientation or any characteristic protected under applicable law.Atalanta will make reasonable accommodations for qualified individuals