In Vivo Neuropharmacology & Molecular Biology Associate

Atalanta Therapeutics, Inc.; Boston (MA); On-site; USD 65,000 - 90,000; Full time

Job summary

Atalanta Therapeutics, Inc. is seeking a Research Associate to join the in vivo pharmacology group.

The role focuses on assay development and execution to characterize biodistribution, pharmacodynamic properties, and efficacy of oligonucleotides in preclinical studies for neurodegenerative disease programs. The candidate will perform molecular and biochemical assays on CNS tissues, develop methods for RNA and protein analysis, and manage tissue samples across rodent and large animal studies,

Qualifications

• Bachelor’s degree or equivalent education with typically 3–5+ years of laboratory working experience, or a Master’s Degree with 0–3+ years experience.
• Hands-on experience in molecular, biochemical and cell biological assays including tissue and cellular DNA/RNA isolation, qRT-PCR, ELISA, in situ hybridization, immunohistochemistry, and cell/tissue imaging by microscopy.
• Hands-on experience in gel electrophoresis and capillary electrophoresis systems such as Bioanalyzer would be preferred.
• Experience in establishing analytical methods for HPLC and Mass Spectrometry would be desirable but not required.
• Experience in CNS tissue harvesting and processing including rodent brain collection, sample collection from large animal brain and spinal cord and tissue homogenization would be preferred
• Experience in animal handling, drug delivery via different routes (IV, IT, stereotactic brain injection, etc.), tissue collection and processing would be desirable but not required.
• Self-motivation, ability to work independently, excellent organization skills with strong attention to details
• Ability to adapt and exercise flexibility for changing platform needs, workflows and projects.
• Strong communication skills (oral and written)

Responsibilities

• Work closely with pharmacology team members to design and execute assays to assess the pharmacological activity, biodistribution and efficacy of oligos in research and preclinical studies to support development of oligonucleotide therapies for neurodegenerative diseases.
• Perform various molecular and biochemical assays on tissue samples from preclinical studies for a variety of CNS drug development programs, including molecular genotyping using PCR assays, quantitative and qualitative analysis of target mRNAs and proteins, quantitative characterization of siRNA from CNS and other tissue matrices, in situ hybridization to characterize mRNA distribution, and transcriptome analysis using RNAseq.
• Manage tissue analysis and oversee the transfer and storage of samples from rodent and large animal studies performed at Atalanta or at external CRO facilities.
• Independent analysis and interpretation of data, careful documentation of experiments and presentation of results within the company

Skills

Molecular assays

Biochemical assays

Cell biology assays

qRT-PCR

ELISA

In situ hybridization

RNAseq analysis

Tissue processing

Data interpretation

Scientific communication

Education

Bachelor's degree

Master's degree

Tools

Bioanalyzer

Job description

Atalanta Therapeutics, Inc. is seeking a Research Associate to join the in vivo pharmacology group.

The role focuses on assay development and execution to characterize biodistribution, pharmacodynamic properties, and efficacy of oligonucleotides in preclinical studies for neurodegenerative disease programs. The candidate will perform molecular and biochemical assays on CNS tissues, develop methods for RNA and protein analysis, and manage tissue samples across rodent and large animal studies,