Senior Research Assistant

Brown University; Providence (RI); On-site; USD 52,000 - 72,000; Full time

Job summary

The Senior Research Assistant at Brown University will support all operations of the RNA Core Facility, including maintaining cell lines, performing transfections, PCR, western blotting, and DNA/RNA/protein extraction and purification. The role includes sample preparation, library construction, and running sequencing workflows under the RNA Core Facility Manager.

This position also handles ordering and preparation of reagents, careful data collection and interpretation, and preparing data for

Qualifications

• Required: Bachelor's degree with 3-5 years' laboratory experience, or equivalent combination of education and experience.
• Knowledge of laboratory approaches and techniques in the areas of Molecular, Cell Biology and Biochemistry required. Candidates should be competent in basic molecular biology techniques, such as PCR, electrophoresis and western blotting, and in growing and maintaining mammalian cell cultures.
• Preferred: Specific experience in RNA/DNA extraction, purification and analysis, nanopore sequencing, biochemistry, or molecular RNA biology.

Responsibilities

• Support all operations of the RNA Core Facility including maintaining cell lines, performing transfections, PCR, western blotting, and DNA/RNA/protein extraction & purification.
• Prepare samples, library construction, and run instrumentation for sequencing and other genomic workflows under the RNA Core Facility Manager.
• Order and prepare necessary laboratory reagents and supplies to support core operations.
• Collect, record and interpret experimental data and prepare data for presentation to outside research groups.
• Write protocols and operating procedures and keep detailed records of activities.

Skills

Molecular biology techniques

Cell culture

Data recording & interpretation

Experimental design & evaluation

Supervision

Communication

Education

Bachelor's degree + 3-5 years lab experience

Tools

Nanopore sequencing

Western blotting

PCR & Gel electrophoresis

Purification & extraction protocols

Job description

Senior Research Assistant

Brown University

Job Description

The Senior Research Assistant will support all operations of theRNA Core Facility including, maintaining cell lines, performingtransfections, PCR, western blotting, and DNA/RNA/proteinextraction & purification. The Senior Research Assistant willbe responsible for the preparation of samples, libraryconstruction, and the running of instrumentation for sequencing andother genomic workflows under the direction of the RNA CoreFacility Manager. In addition, the Senior Research Assistant willbe responsible for the ordering and preparation of necessarylaboratory reagents and supplies to support Core operations. Theposition will require careful collection, recording, andinterpretation of experimental data and preparation of collecteddata for presentation to outside research groups. The position willrequire the ability to keep detailed records of activities, andwill be required to write protocols and operating procedures.

The COBRE: Center for RNA Biology in Health and Disease primarygoal is to create a transformative organizing hub for RNA researchin Rhode Island. The RNA Core Facility works to support RNA biologyresearch at Brown University and the wider Rhode Island communityby providing specialized expertise and training. The RNA CoreFacility is developing analytical capabilities in long-readsequencing, RNA immunoprecipitation, massively parallel reporterassays (MRPAs), and RNA structural analysis. We work with earlycareer RNA researchers to help them establish independentNIH-funding investigators, more established researchers who areseeking to initiate RNA-focus projects and act to fosterinterdisciplinary collaboration between core services and RNAbiology research labs.

This is a fixed term position with a current end date of one yearfrom the date of hire which may be extended based upon availablefunding.

JOB QUALIFICATIONS

• Required: Bachelor's degree with 3-5 years' laboratoryexperience, or equivalent combination of education andexperience
• Knowledge of laboratory approaches and techniques in the areasof Molecular, Cell Biology and Biochemistry required. Candidateshould be competent in basic molecular biology techniques, such asPCR, electrophoresis and western blotting, and in growing andmaintaining mammalian cell cultures.
• Preferred: Specific experience in RNA/DNA extraction,purification and analysis, nanopore sequencing, biochemistry, ormolecular RNA biology.

Job Competencies

• Strong organizational skills, technical/scientific and timemanagement skills
• Ability to supervise others
• Ability to design, conduct and evaluate experimentsindependently
• Knowledge of molecular biology techniques
• Ability to communicate clearly and professionally withco-workers
• Ability to interact and collaborate with diverse researchgroups and other core facilities

CRIMINAL BACKGROUND CHECK AND EDUCATION VERIFICATIONREQUIRED

Benefits of Working at Brown:

Please review additional information on the Benefits of Working atBrown.

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Brown University provides equal opportunity and prohibitsdiscrimination, harassment and retaliation based upon a person'srace, color, religion, sex, age, national or ethnic origin,disability, veteran status, sexual orientation, gender identity,gender expression, or any other characteristic protected underapplicable law, in the administration of its policies, programs,and activities. The University recognizes and rewards individualson the basis of qualifications and performance. The Universitymaintains certain affirmative action programs in compliance withapplicable law.