Senior Protein Scientist: Bioconjugation & Library Prep

Nautilus Biotechnology; San Carlos (CA); On-site; USD 180,000 - 260,000; Full time

Job summary

Nautilus Biotechnology is seeking a seasoned protein scientist to lead the protein handling and bioconjugation workstreams for a new sample preparation workflow on our proteomics platform.

You will own library prep end-to-end, design multi-factor experiments, and ensure data-driven go/no-go decisions, collaborating with assay development, probe development, and bioinformatics teams to deliver a robust, documented protocol for other teams to run.

Qualifications

• PhD in biochemistry, chemistry, chemical biology, bioengineering, or a related field with 10+ years of industry experience; or BS/MS with 15+ years in a related field
• Deep hands-on expertise handling, purifying, and stabilizing proteins
• Demonstrated record of innovation in protein bioconjugation (e.g., site-selective labeling, click chemistry, crosslinkers, surface immobilization)
• Expert in protein characterization: mass spectrometry, SEC, DLS, CD, SDS-PAGE/native PAGE, and activity or binding assays
• Proven ability to take a workflow from concept to a robust, reproducible protocol used by other teams
• Exceptional experimental design and data analysis skills, including multi-factor experiments
• Track record of leading cross-functional technical work and influencing without authority
• Experience mentoring scientists and setting technical direction for a team

Responsibilities

• Own the proteomics sample preparation workflow end to end, from strategy and first experiments to a robust protocol others can run
• Lead development of bioconjugation strategies to label and immobilize proteins with minimal impact on protein integrity
• Build the characterization toolkit for every step: prove what's conjugated, what's intact, what's aggregated, and what was lost
• Set quality metrics and acceptance criteria for prepared protein samples libraries, and use them to make go/no-go calls
• Design complex, multi-factor experiments that give the program decisive direction
• Drive root cause and permanent corrective actions when the workflow fails
• Partner with assay, probe development, and bioinformatics teams to integrate library prep into the full workflow and interpret results together
• Present plans, results, and recommendations to the team and to R&D leadership
• Set technical direction for library prep, mentor scientists and associates, and weigh in on program-level tradeoffs

Skills

Protein handling

Bioconjugation

Site-specific labeling

Protein immobilization

Experimental design

Cross-functional collaboration

Data analysis

Mentoring

Education

PhD in biochemistry/chemistry/chemical biology/bioengineering or related

BS/MS with 15+ years in related field

Tools

Mass spectrometry

SEC

DLS

CD

SDS-PAGE/native PAGE

Activity or binding assays

Job description

Nautilus Biotechnology is seeking a seasoned protein scientist to lead the protein handling and bioconjugation workstreams for a new sample preparation workflow on our proteomics platform.

You will own library prep end-to-end, design multi-factor experiments, and ensure data-driven go/no-go decisions, collaborating with assay development, probe development, and bioinformatics teams to deliver a robust, documented protocol for other teams to run.