Scientist-Senior Scientist- Protein Chemistry

OneDigital NorCal HR Consulting; Seattle (WA); On-site; USD 77,000 - 96,000; Full time

Job summary

A stealth biotech company in Seattle is seeking a highly motivated Scientist or Senior Scientist. Responsibilities include protein expression, purification, and characterization, focusing on protein-protein interactions and biophysical techniques. The ideal candidate will have experience with antibodies and bispecifics, contributing to innovative therapeutic protein development.

Benefits

Competitive compensation package

Performance bonuses

Equity options

Comprehensive health benefits

Opportunities for professional growth

Qualifications

• 3-5 years of relevant industry experience.
• Hands-on experience with biophysical techniques.

Responsibilities

• Design constructs for recombinant protein expression.
• Optimize protocols for high-yield protein production.
• Manage external collaborations with CROs.

Skills

Problem-Solving

Organizational Skills

Education

Ph.D. in Biochemistry

M.S. or B.S. degree

Tools

BLI

SPR

SDS-PAGE

Western Blotting

UV-Vis Spectroscopy

Dynamic Light Scattering

Job description

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A small stealth biotech company in downtown Seattle is seeking a highly motivated Scientist or Senior Scientist to join our dynamic team. The ideal candidate will be responsible for protein expression, purification, characterization, and analysis, with a focus on protein-protein interactions, biophysical techniques (e.g., BLI, SPR), and coordination of internal and external (CRO) work. Prior experience with antibodies and bispecifics is highly desirable.

Key Responsibilities

Protein Expression and Purification:

• Design constructs for recombinant protein expression in bacterial, insect, or mammalian systems.
• Optimize protocols for high-yield and high-purity protein production.
• Perform purification using techniques such as affinity chromatography, ion exchange (IEX), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC).

Protein Characterization and Analysis:

• Conduct biophysical studies using BLI (Bio-Layer Interferometry) and SPR (Surface Plasmon Resonance) to study protein-protein interactions.
• Perform quality control assays including SDS-PAGE, Western blotting, UV-Vis spectroscopy, and dynamic light scattering.
• Analyze protein stability and aggregation using advanced techniques like SEC-MALS or differential scanning fluorimetry.

Antibody and Bispecific Development:

• Express and purify monoclonal antibodies and bispecific molecules.
• Characterize binding affinities and functional properties of antibody-based therapeutics.

Coordination with CROs:

• Manage external collaborations with Contract Research Organizations (CROs) for protein production and characterization.
• Troubleshoot challenging proteins and ensure timely delivery of results.

Cross-functional Collaboration:

• Work closely with interdisciplinary teams including structural biologists, assay development scientists, and therapeutic project leads.
• Communicate progress, challenges, and results effectively to internal stakeholder.

Qualifications

Education:

• Ph.D. in Biochemistry, Structural Biology, Biophysics, or related field with at least 3–5 years of relevant industry experience.
• Candidates with an M.S. or B.S. degree and significant hands-on experience will also be considered.

Technical Expertise:

• Proven track record in recombinant protein expression, purification, and characterization.
• Hands-on experience with biophysical techniques such as BLI or SPR is required.
• Familiarity with antibody engineering or bispecific design is strongly preferred.

Skills:

• Strong problem-solving skills for troubleshooting complex protein-related challenges.
• Excellent organizational skills with attention to detail for documentation in electronic lab notebooks (ELN).
• Ability to work independently as well as collaboratively in a fast-paced environment.

Additional Desired Skills:

• Experience in managing external collaborations (e.g., CROs).
• Knowledge of structural biology methodologies such as X-ray crystallography or cryo-EM is a plus.
• Familiarity with automation tools for protein purification is advantageous.

The company offers a competitive compensation package including salary, performance bonuses, equity options, comprehensive health benefits, and opportunities for professional growth in a collaborative environment.

This role provides an exciting opportunity to contribute to the development of innovative therapeutic proteins in a rapidly growing biotech company.

Seniority level

• Seniority level

  Mid-Senior level

Employment type

• Employment type

  Full-time

Job function

• Job function

  Science

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