Scientist, Protein Technologies: CRISPR & Stable Cell Lines
Thecentermemphis
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Job summary
St. Jude Children's Research Hospital is seeking a Scientist in the Protein Technologies Center to advance genome editing and protein tagging efforts for structural biology and drug discovery.
The role involves editing cell lines, verifying genomic stability, and supporting multidisciplinary projects within the department. The candidate will employ CRISPR and cloning technologies to create gene knockouts/knock-ins and build stable expression platforms, contributing to cell engineering and
Qualifications
- Bachelor's degree in relevant scientific area required.
- Eight (8) years of relevant work experience with a Bachelor's degree; Six (6) years with a Master's; Three (3) years with a PhD.
- PhD preferred (with at least 5 years of postdoctoral experience) in molecular biology or synthetic biology or related discipline.
Responsibilities
- Engineer cell lines using CRISPR gene editing for knockouts and knock-ins and confirm edits.
- Generate stable cell lines using non-viral and viral delivery systems.
- Develop stable target-overexpressed cell lines, pools, clones, and perform NGS library preparation.
- Demonstrate proven cloning ability with multiple platforms; manage large/complex DNA constructs and libraries.
Skills
Education
Tools
Job description
St. Jude Children's Research Hospital is seeking a Scientist in the Protein Technologies Center to advance genome editing and protein tagging efforts for structural biology and drug discovery.
The role involves editing cell lines, verifying genomic stability, and supporting multidisciplinary projects within the department. The candidate will employ CRISPR and cloning technologies to create gene knockouts/knock-ins and build stable expression platforms, contributing to cell engineering and
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