CRISPR Protein Engineer & Cell Line Scientist

St. Jude Children's Research Hospital; Memphis (TN); On-site; USD 86,320 - 154,960; Full time

Benefits offered by this job

Medical plan

Dental plan

Vision plan

Retirement plan

Wellness program

Time off programs

Educational benefits

Family benefits

St. Jude benefits

Job summary

St. Jude Children's Research Hospital's Protein Technologies Center seeks a scientist skilled in saturation-mutagenesis reinforced functional assays, site-specific integrase-based stable expression platforms, and genome editing to tag endogenous proteins with fluorescent and affinity tags for structural, functional, and drug-discovery studies.

The role is bench-based, involving CRISPR editing of cell lines, verification of edits, and maintenance of genomic stability across clonal line generation

Qualifications

• Bachelor's degree in relevant scientific area is required.
• PhD preferred with at least 5 years postdoctoral experience.
• Extensive experience in molecular biology and synthetic biology applicable to protein engineering.

Responsibilities

• Engineer cell lines using CRISPR gene editing and confirm edits.
• Generate stable cell lines using non-viral and viral delivery systems.
• Develop stable target-overexpressed cell lines, pools, and clones; prepare NGS libraries.
• Handle complex DNA constructs and perform site-directed or saturation mutagenesis.

Tools

CRISPR gene editing

Non-viral delivery systems

Viral delivery systems

NGS library preparation

DNA construct handling

Molecular cloning methods

Job description

St. Jude Children's Research Hospital's Protein Technologies Center seeks a scientist skilled in saturation-mutagenesis reinforced functional assays, site-specific integrase-based stable expression platforms, and genome editing to tag endogenous proteins with fluorescent and affinity tags for structural, functional, and drug-discovery studies.

The role is bench-based, involving CRISPR editing of cell lines, verification of edits, and maintenance of genomic stability across clonal line generation