Scientist l, Cell Line Development (based fully onsite)

BioSpace; South San Francisco (CA); On-site; USD 110,000 - 150,000; Full time

Job summary

AbbVie in South San Francisco, CA seeks a Scientist I to join the Cell Line Development group. You will execute CHO cell line development and characterization for GMP applications, owning projects end-to-end and collaborating across process and analytical development.

The role covers transfection, clone screening, culture scale-up, DNA/RNA extraction, qPCR/ddPCR, and assay support, with emphasis on data integrity and timely delivery in a fast-moving environment.

Qualifications

• Bachelors Degree or equivalent education with typically 5+ years of experience, or Masters Degree with 2+ years in biologics discovery or development.
• Hands-on experience in aseptic technique and mammalian cell culture, focusing on CHO cells, managing multiple cell lines and projects in parallel.
• Proficiency in nucleic acid extraction from mammalian cells and PCR-based analysis including qPCR and/or ddPCR.
• Experience developing stably transfected CHO cell lines to produce monoclonal antibodies or antibody-like molecules, including transfection, selection, mini-pool isolation, clone isolation and screening, and scale-up.

Responsibilities

• Experimental design and interpretation; perform routine and complex lab tasks and propose follow-up experiments.
• Execute CHO cell line development workflows for pipeline and innovation projects including transfection and clone screening.
• Molecular characterization using PCR/qPCR/ddPCR; support vector design and sequence confirmation for antibodies.
• Single-cell cloning and cryopreservation of research cell banks; maintain inventory records.
• Design and run fed-batch and parallel culture studies to evaluate clone productivity, growth, and stability.
• Operate and maintain analytical instrumentation (e.g., Vi-CELL) and culture equipment.
• Develop and optimize new methods and assays to generate reliable data; document protocols and train team members.
• Contribute to qualification and troubleshooting of assays; identify opportunities to improve throughput and data quality.

Skills

Aseptic technique

Mammalian cell culture

PCR/qPCR/ddPCR

Cell line development

Data interpretation

Cross-functional collaboration

Education

Bachelor's degree or equivalent

Master's degree or equivalent

Tools

Octet

Transfection methods

Cell culture instrumentation

Job description

About AbbVie

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us atwww.abbvie.com. Follow @abbvie onLinkedIn,Facebook,Instagram,XandYouTube.

Company Description

About AbbVie

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us atwww.abbvie.com. Follow @abbvie onLinkedIn,Facebook,Instagram,XandYouTube.

Job Description

The Cell Line Development group in South San Francisco, CA is seeking a Scientist I to join a talented and collaborative team developing CHO cell lines for GMP applications. In this hands-on laboratory position, you will execute CHO cell line development and characterization studies in a well-supported, fast-moving environment that advances novel biotherapeutics into human clinical studies. The work spans molecular, cellular, and bioprocess development activities supporting the generation and characterization of optimized CHO cell lines for ourbiologicspipeline candidates.

This is a bench-focused position on a small team where each scientist owns their projects end to end and works directly with partners across process and analytical development. Our group runs both platform and non-platform molecules, so the work varies considerably from project to project.

Responsibilities

• Experimental design and interpretation.Perform both routine and complex laboratory tasks competently and independently, generating reliable and consistent results. Independently design and conduct critical experiments that further project goals. Interpret results and draw conclusions from your own multistage experiments, note significant deviations, and then suggest, design, and pursue the follow-up experiments those results call for.
• Cell line development execution.Execute CHO cell line development workflows for pipeline and innovation projects, including transfection, selection, high-throughput clone screening, and culture scale-up from multi-well plates through shake flasks and small-scale stirred tank vessels. Apply both random and targeted integration approaches, including transposase- and recombinase-mediated systems.
• Molecular and genetic characterization.Extract DNA and RNA from CHO cell lines and cell banks and perform genetic characterization by PCR, qPCR/RT-qPCR, ddPCR, and sequencing-based methods. Support vector design, construction, and sequence confirmation for antibodies and novel protein formats.
• Clone generation and cell banking.Perform single-cell cloning to establish monoclonal CHO cell lines. Cryopreserve research cell banksand maintain accurate bank inventory records.
• Productivity and bioprocess assessment.Design and run fed-batch and parallel culture studies, on both manual and automated platforms, to evaluate clone productivity, growth, and stability.
• Instrumentation.Operate and maintain the group's analytical and cell culture instrumentation, including automated cell counters (Vi-CELL) and label-free binding platforms (Octet).
• Method development and assay support.Investigate, identify, develop, and optimize new methods and techniques to generate reliable and reproducible data in a timely manner, reading and adapting the scientific literature to accomplish assignments and demonstrating broad and versatile technical expertise within the discipline.
• Contribute to the qualification and troubleshooting of the assays that underpin clone selection, implement and where possible innovate new experimental protocols, and identify opportunities to improve throughput, data quality, and overall workflow efficiency.
• Documentation and knowledge transfer.Maintain meticulous, contemporaneous records of experimental design, data, and analysis in electronic lab notebooks (ELNs), in compliance with AbbVie's standards for data integrity and good documentation practices. Author and reviseprotocols, andhelp train new team members on cell culture and characterization techniques.
• Cross-functional collaboration and communication.Present scientific updates at team and cross-functional meetings and partner closely with colleagues in process developmentand analytical groupsto drive projects forward and meet aggressive timelines.
• Compliance and safety.Understand and adhere to corporate standards regarding code of conduct, safety, the appropriate handling of materials, andGxPcompliance.

Qualifications

• Bachelors Degree or equivalent education with typically 5+ years of experience, or Masters Degree or equivalent education with typically 2+years of experiencein biologics discovery or development, or a closely related discipline.
• Hands-on experience in aseptic technique and mammalian cell culture, with a primary focus on CHO cells, including the ability to manage multiple cell lines and projects in parallel.
• Proficiency in nucleic acid extraction from mammalian cells and PCR-based analysis, including qPCR and/or ddPCR.
• Hands-on experience developing stably transfected CHO cell linesto producemonoclonal antibodies or antibody-like molecules, including transfection,selection,minipool isolation,clone isolation and screening, and scale-up.
• Hands-on experience with transposase/recombinase technologies in CHO cell line development.
• Practical experience cryopreserving CHO cells and generating research cell banks, including assessment of post-thaw viability and recovery, and accurate inventory record-keeping.
• Experience quantifying recombinant protein titer by biolayer interferometry (e.g., Octet) or an equivalent titer method.
• Experience running plate-based screening assays to rank large numbers of clones in 96-well and 24-deep-well plate formats, including plate mapping, scheduled sampling, and review of the resulting data for outliers and plate effects.
• Experience evaluating clone productivity, growth, and stability in batch or fed-batch culture, including scheduled sampling, data analysis, and ranking of candidate clones on manual and/or automated platforms.
• Demonstrated ability to independently design, execute, interpret, and troubleshoot experiments that answer defined scientific questions and advance project goals, including sound analysis of quantitative data, the judgment to recognize significant deviations, and the initiative to design the follow-up experiments they call for.
• Strong understanding of mammalian cell biology and molecular biology concepts, with the ability to draw on relevant scientific literature and apply theoretical frameworks to solving problems within the discipline in a timely manner.
• Excellent attention to detail, with a strong commitment to maintaining detailed, daily records in an electronic lab notebook.
• Strong written and verbal communication skills, including the ability to present data clearly to colleagues and cross-functional partners.

Additional Information

• The compensation range described below is the range of possible base pay compensation that the Companybelieves ingood faith it will pay for this role at thetimeofthis posting based on the job grade for this position.Individualcompensation paid within this range will depend on many factors including geographiclocation,andwemayultimatelypaymore or less than the posted range. This range may bemodifiedin thefuture.
• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick),medical/dental/visioninsurance and 401(k) to eligibleemployees.
• This job is eligible toparticipatein our short-term incentiveprograms.

Note: No amount of payis considered to bewages or compensation until such amount is earned, vested, anddeterminable.Theamountandavailabilityof anybonus,commission, incentive, benefits, or any other form ofcompensation and benefitsthat are allocable to a particular employeeremainsin the Company'ssoleandabsolutediscretion unless and until paid andmay bemodifiedat the Companys sole and absolute discretion, consistent withapplicable law.

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives and serving our community. Equal Opportunity Employer/Veterans/Disabled.

US & Puerto Rico only - to learn more, visithttps://www.abbvie.com/join-us/equal-employment-opportunity-employer.html

US & Puerto Rico applicants seeking a reasonable accommodation, click here to learn more:

https://www.abbvie.com/join-us/reasonable-accommodations.html