Scientist I, Multi-Specifics Expression

BioSpace; North Chicago (IL); On-site; USD 120,000 - 180,000; Full time

Benefits offered by this job

Paid time off

Medical/dental/vision insurance

401(k)

Short-term incentive programs

Job summary

AbbVie Biotherapeutics and Genetic Medicine is seeking an experienced protein expression scientist to join a fast-paced, high-throughput CHO team. You will support expression and characterization of complex multi-specific biologics and scale from 1 to 200 L for drug discovery.

The role emphasizes hands-on CHO culture, vector design proficiency, and collaboration with cross-functional teams. You will troubleshoot expression issues and contribute to data-driven decision making across discovery

Qualifications

• BS or MS in biology, chemistry, biochemistry or related field.
• 5+ (BS) or 2+ (MS) years of experience in relevant roles.
• Hands-on CHO cell culture and expression of antibodies/multi-specifics.
• Experience with stable CHO cell lines expressing complex multi-species proteins.
• Process development, optimization, and automation experience.
• Analytical characterization of multi-specifics desirable.
• Targeted integration, FACS, and ddPCR desirable.

Responsibilities

• Expression of novel multi-specific biologics formats for drug discovery.
• Generate and screen stable CHO cell lines with optimized attributes.
• Determine optimal chain ratios for multi-chain molecules.
• Process development for batch and fed-batch expressions in CHO.
• Characterize CHO line copy number and mRNA levels.
• Apply screening workflows and single-cell sorting to mini pools.
• Implement automation platforms and high-throughput workflows.
• Contribute to data digitization, analysis, and reporting.
• Troubleshoot expression, stability, glycosylation, and aggregation.
• Purification and biophysical characterization of samples.
• Work independently on design, execution, and interpretation of results.
• Document and report data concisely and rigorously.

Skills

Cell culture

Mammalian expression

Antibody expression

FACS

ddPCR

Automation

Data analysis

Education

Bachelor's or Master's in biology

Tools

Wave bioreactors

Job description

About AbbVie

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us atwww.abbvie.com. Follow @abbvie onLinkedIn,Facebook,Instagram,XandYouTube.

Company Description

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us atwww.abbvie.com. Follow @abbvie onLinkedIn,Facebook,Instagram,XandYouTube.

Overview

Job Description

AbbVie Biotherapeutics and Genetic Medicine is an integrated, global discovery organization responsible for biotherapeutics drug research across disease areas including immunology, oncology and neuroscience. Our scope includes the discovery of innovative biologic medicines from the early exploratory stage and their support through clinical development. We are growing a world-class team of scientists and leaders who will drive the discovery and advancement of innovative biologic-based therapies. Careers in AbbVie Biotherapeutics and Genetic Medicine provide unique opportunities to work with cross-functional teams that drive exceptional science and deliver innovative medicines to patients. We are looking for diverse candidates with strong scientific and leadership experience to join the team and can offer exciting and rewarding career opportunities for these candidates.

The Biotherapeutics Production group at AbbVie is seeking an experienced protein expression scientist to join a fast paced, high throughput, large scale mammalian expression group. This role will focus on CHO cell line engineering biologics, protein expression, and characterization of complex biotherapeutics, including multi-specific, while incorporating modern capabilities in automation and data digitization to enable efficient and scalable workflows. The Biotherapeutics mammalian expression group supports research scale (1 to 200L) expression of biologics in support of AbbVies worldwide drug discovery efforts.

Key Responsibilities

• Expression of novel multi-specific biologics formats to support therapeutic area drug discovery projects.
• Generation and screening of stable CHO cell lines with optimized attributes (i.e. titer, monomer, chain-pairing) using 2 or more vectors.
• Determine optimal chain ratio for 3- and 4-chain molecules in CHO cells using multiple selection strategies and process optimization.
• Process development and optimization for batch and fed-batch bioreactor and shake flask expressions of stable CHO cell lines expressing complex multi-chain molecules.
• Characterization of stable CHO line copy number and mRNA transcript levels using ddPCR and related molecular tools.
• Apply targeted integration technologies using screening workflows, including FACS-based single-cell sorting to generate mini pools.
• Implement and leverage automation platforms and high-throughput workflows to increase efficiency.
• Contribute to data digitization efforts, including electronic data capture, analysis, and reporting.
• Troubleshoot challenges related to expression, stability, glycosylation, aggregation, and chain pairing.
• Purification and biophysical characterization of analytical scale expression samples, using analytical HPLC to evaluate titer, monomer, and chain pairing efficiency.
• Work independently on the design, execution, and interpretation of experimental results.
• Analyze, document, and report data concisely and rigorously.
• Handling stable CHO pools and cell lines including thaw, culture, expression and cryopreservation.

Qualifications

• Position will be hired based on level of experience and education
• Bachelors or Masters in biology, chemistry, biochemistry or related field with typically 5+ (BS) or 2+ (MS) years of experience.
• Hands-on experience with cell culture and expression of monoclonal antibodies, bi-specifics and multi-specifics in CHO cells lines including thaw, culture, expression and cryopreservation.
• Prior experience in generation of stable CHO cell lines expressing complex multi-specific molecules.
• Prior experience in process development, process optimization, and automation.
• Experience with analytical characterization of multi-specifics desirable.
• Experience with targeted integration, FACS, and ddPCR desirable.
• Demonstrated proficiency in vector design is desirable.
• Prior experience in mammalian expression using Wave bioreactors desirable.
• Demonstrated proficiency in overcoming problems (i.e. poor chain pairing, low expression, aggregation, glycosylation) with challenging therapeutic multi-specific proteins desirable.

Additional Information

• The compensation range described below is the range of possible base pay compensation that the Companybelieves ingood faith it will pay for this roleat thetimeofthis posting based on the job grade for this position.Individualcompensation paid within this range will depend on manyfactors including geographiclocation,andwemay ultimately pay more or less than the posted range. This range may bemodified in thefuture.
• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick),medical/dental/visioninsurance and 401(k) to eligibleemployees.
• This job is eligible toparticipate in our short-term incentiveprograms.

Note: No amount of payis considered to bewages or compensation until such amount isearned, vested, anddeterminable.Theamountandavailabilityof anybonus,commission, incentive, benefits, or any other form ofcompensation and benefitsthat areallocable to a particular employeeremains in the Company'ssoleandabsolutediscretion unless and until paid andmay bemodified at the Companys sole and absolutediscretion, consistent withapplicable law.

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives and serving our community. Equal Opportunity Employer/Veterans/Disabled.

US & Puerto Rico only - to learn more, visit https://www.abbvie.com/join-us/equal-employment-opportunity-employer.html

US & Puerto Rico applicants seeking a reasonable accommodation, click here to learn more: https://www.abbvie.com/join-us/reasonable-accommodations.html