Scientist III Cell Biology & Functional Genomics

eTeam; Ridgefield (CT); On-site; USD 110,000 - 140,000; Full time

Job summary

eTeam is seeking a Scientist III to support discovery research in cardiovascular-renal-metabolic areas. The role focuses on hands-on mammalian cell culture, cell-based assays, and functional genomics, including NGS-focused workflows and iPSC-derived processes.

Responsibilities include operating automated workflows, library preparation for RNA-seq, and cross-team collaboration to deliver robust data and insights.

Qualifications

• MS degree with 3+ years relevant experience or BS with 7+ years in cell biology, genomics, or related field.
• Experience in mammalian cell culture, cell biology, and cell-based assays.
• Strong documentation and data integrity practices.
• Ability to troubleshoot routine experiments independently.
• Experience with NGS workflows and sequencing library prep is a plus.

Responsibilities

• Design, execute, and analyze cell-based experiments for target validation and discovery.
• Operate and monitor automated workflows for cell-based and molecular assays; troubleshoot issues.
• Perform dissociation and prepare libraries for bulk RNA-seq and single-cell RNA-seq workflows.
• Conduct molecular biology techniques (DNA/RNA extraction, PCR, qPCR, western blot).
• Maintain accurate ELN/reports and ensure SOP/compliance across experiments.
• Collaborate with biology, automation, genomics, and bioinformatics teams.

Skills

Mammalian cell culture

NGS workflows

Laboratory automation

Attention to detail

Troubleshooting

Education

MS + 3 yrs

BS + 7 yrs

Equivalent experience

Tools

Laboratory automation platforms

NGS platforms

Job description

Job Title: Scientist III Cell Biology & Functional Genomics

Location: Ridgefield, CT

Duration: 12 Months

Job Description

The successful candidate will support discovery research programs within Cardiovascular-Renal-Metabolic Diseases through hands-on operation of laboratory automation platforms, mammalian cell culture, cell-based assays, and functional genomics studies. The candidate will execute and monitor established automated cell culture and assay workflows and support automated iPSC-derived kidney organoid processes and next-generation sequencing (NGS)-related work, with platform- and workflow-specific training provided as needed.

The role requires reliable execution of automation and NGS workflows. Success requires exceptional attention to detail, careful sample and reagent tracking, precise adherence to established protocols, and independent troubleshooting of routine experimental or platform issues.

Duties & Responsibilities

• Design, execute, and analyze cell-based experiments supporting target validation and discovery research, including routine mammalian cell culture, assay execution, image-based analysis, and iPSC-derived cell workflows.
• Operate and monitor established laboratory automation workflows for cell-based and molecular assays, troubleshoot routine issues, and elevate complex problems appropriately.
• Perform tissue, cell, and nuclei dissociation and execute NGS workflows for bulk RNA-seq, single-cell RNA-seq, single-nucleus RNA-seq, and related applications, including library preparation, quality control, quantification, normalization, sequencing setup, and sequencer operation.
• Perform routine molecular biology techniques, including DNA/RNA extraction, PCR and qPCR, nucleic acid and protein quantification, Western blotting, and basic sample preparation; analyze and interpret results, identify technical issues, and communicate findings and recommended follow-up clearly.
• Maintain accurate sample, reagent, consumable, experimental metadata, and electronic laboratory notebook records while complying with SOPs, safety requirements, company policies, and data integrity standards.
• Collaborate across cell biology, automation, genomics, and bioinformatics groups.

Skills

• Strong hands-on experience with mammalian cell culture, cell biology, and cell-based assay execution.
• Hands-on NGS workflow experience, including tissue, cell, and/or nuclei dissociation, sequencing library preparation, quality control, and quantification for bulk RNA-seq, single-cell RNA-seq, single-nucleus RNA-seq, or related applications.
• Some hands-on experience with laboratory automation, automated instrumentation, or standardized high-throughput workflows, with the ability and interest to develop further expertise in operating and troubleshooting automated cell-based and molecular assays; automation programming is not required.
• Exceptional attention to detail and demonstrated ability to accurately execute lengthy, multi-step protocols while maintaining sample integrity, data quality, and complete documentation.
• Ability to independently troubleshoot routine experimental or operational issues, communicate clearly, and work effectively in a collaborative research environment.

Desired Skills

• Experience running laboratory automation platforms for cell-based or molecular assays.
• Experience with sequencing run setup and sequencer operation. Instrument-specific operation can be trained when required.
• Experience with human induced pluripotent stem cell culture, differentiation, characterization, or iPSC-derived cell assays.
• Experience with kidney organoid culture systems.

Education

• Master's degree with 3 years of relevant experience; or
• Bachelor's degree with 7 years of relevant experience; or
• Equivalent combination of education and experience in cell biology, molecular biology, genomics, or a related scientific discipline.