Scientist III

Intellectt INC; Worcester (MA); On-site; USD 90,000 - 125,000; Full time

Job summary

Intellectt INC is seeking a Scientist III in Worcester, MA for a 06+ month role focused on advanced protein analytics and purification. You will perform HPLC/UPLC, LC-MS, and analytical method development to support biologics characterization, PTM analysis, and data documentation.

The position requires hands-on experience with chromatographic separation, protein mass spectrometry, and cross-functional collaboration within a quality-driven environment.

Qualifications

• Bachelor's or Master's in a relevant field with 5+ years of experience
• Experience in protein/biologic analytical development
• Proficiency with chromatography-based separation and LC-MS analysis

Responsibilities

• Perform protein separation and fractionation using chromatography systems
• Characterize protein variants and analyze fractions
• Perform LC-MS including intact/reduced protein mass analysis and peptide mapping
• Support PTM identification and sequence verification
• Document experiments and results in ELN
• Maintain and troubleshoot HPLC/UPLC/KTA systems

Skills

HPLC/UPLC experience

Protein purification

LC-MS / protein MS

Biologics / large molecules

Analytical method development

Documentation in ELN

Cross-functional collaboration

Education

Bachelor's degree in Chemistry/Biology/Biochemistry

Master's degree in Chemistry/Biology/Biochemistry

Tools

KTA systems

HPLC instruments

UPLC instruments

LC-MS instrumentation

Job description

Job Title: Scientist III

Location: Worcester, MA

Duration: 06+ Months

Key Responsibilities:

• Perform protein separation and fractionation using KTA, HPLC, UPLC, and related chromatography systems
• Characterize protein size and charge variants and analyze protein fractions
• Perform LC-MS including intact/reduced protein mass analysis and peptide mapping
• Support sequence verification and post-translational modification (PTM) identification
• Perform biophysical characterization including DSC
• Design and execute analytical method development and investigation experiments
• Support raw material analysis using IR, NIR, Raman, or other vibrational spectroscopy
• Support mass spectrometry-based metabolomics studies
• Optimize analytical methods and troubleshoot instruments and analytical assays
• Generate reliable and reproducible analytical data
• Document experiments and results in ELN
• Prepare protocols, method development reports, and technical characterization reports
• Maintain and troubleshoot KTA, HPLC, and laboratory equipment
• Collaborate with cross-functional teams while following safety, quality, and corporate standards

Must Have Skills:

• Strong hands-on HPLC, UPLC, and liquid chromatography experience
• Hands-on KTA or similar protein purification/separation experience
• Protein separation and analytical characterization
• Biologics / large-molecule analysis
• LC-MS / protein mass spectrometry
• Protein structure and PTM knowledge
• Analytical separation techniques
• Analytical method development, optimization, and troubleshooting
• Strong communication, documentation, organization, and multitasking skills

Nice To Have Skills:

• CE-SDS, icIEF, or CZE
• Protein biophysical characterization and DSC
• Waters or Agilent HPLC systems
• Intact/reduced protein mass analysis
• Peptide mapping by LC-MS
• Bioinformatics tools for mass spectrometry data analysis
• IR, NIR, or Raman spectroscopy
• Multivariate analysis
• Mass spectrometry-based metabolomics
• Analytical investigations

Education & Experience:

• Bachelor's Degree: Chemistry, Biology, Biochemistry, or related technical discipline with typically 5+ years of relevant experience
• Master's Degree: Chemistry, Biology, Biochemistry, or related technical discipline with typically 2+ years of relevant experience

Required Experience:

• Protein / biologics analytical development
• Chromatography-based protein separation and analysis
• HPLC / UPLC and KTA systems
• LC-MS / protein mass spectrometry
• Biologics / large-molecule analytical characterization
• Analytical method development, optimization, and troubleshooting