Scientist II (Viral Vector)

BEPC Inc. - Business Excellence Professional Consulting; South San Francisco (CA); Vor Ort; USD 59.000 - 67.000; Vollzeit

Benefits dieser Stelle

Medical insurance

Dental insurance

Vision insurance

Life insurance

Zusammenfassung

BEPC Inc. in South San Francisco, CA is seeking an experienced Scientist II (Viral Vector) for a 6-month W2 contract to support LVV and AAV production, process development, and automation initiatives.

The role emphasizes ex vivo CAR T-cell engineering using lentiviral vectors, mammalian cell culture, and molecular biology techniques. Ideal candidates hold an MS or PhD with 3+ years of relevant experience, strong hands-on lab skills, and the ability to design experiments (DOE) and collaborate

Qualifikationen

• MS or PhD in Cell Biology, Molecular Biology, Virology, Biochemistry, Bioengineering, Genetics, or related field.
• 3+ years of laboratory research or industrial experience.
• Hands-on experience with ex vivo CAR T-cell engineering using lentiviral vectors is required.
• Strong proficiency in mammalian cell culture and molecular biology techniques.
• Experience with PCR, qPCR, ddPCR/dPCR, and Western blot.

Aufgaben

• Develop and optimize LVV and AAV production workflows to improve yield and throughput.
• Automate high-throughput workflows using liquid handling systems.
• Optimize LVV upstream production including cell expansion and transfection.
• Apply DOE to optimize plasmid ratios and production parameters.
• Develop downstream purification processes (filtration, chromatography).
• Support high-throughput production using automated bioreactors (Ambr 15/250).
• Perform viral vector QC and characterization assays.
• Analyze experimental data and maintain ELNs and sample traceability.
• Prepare SOPs and reports; present findings to teams.

Kenntnisse

Molecular biology

Ex vivo CAR T-cell engineering

Mammalian cell culture

PCR/qPCR/ddPCR/dPCR

Data analysis (Excel/GraphPad)

DOE/experimental design

Lab automation understanding

Ausbildung

MS or PhD in a related field

Tools

Integra Assist Plus

Bravo liquid handling system

Ambr 15/Ambr 250

TFF/Filtration systems

Chromatography

Flow cytometer

Jobbeschreibung

Employment Type: W2 Contract – 6 Months with Possibility for Extensions

Pay Rate: $43.00–$48.96/hour – Based on experience (Paid Weekly)

Benefits: Medical, Dental, Vision, and Life Insurance

Note: This is a W2 only role — C2C and C2H will not be considered.

Role Overview

BEPC is seeking an experienced Scientist II (Viral Vector) to support our client's innovative research and development programs in South San Francisco, CA.

This position focuses on lentiviral vector (LVV) and adeno-associated virus (AAV) production, upstream and downstream process development, and laboratory automation to improve viral vector yield, throughput, and quality.

The ideal candidate has an MS or PhD with 3+ years of relevant experience, strong molecular biology expertise, and hands-on experience with ex vivo CAR T-cell engineering using lentiviral vectors.

Key Responsibilities

• Develop and optimize LVV and AAV production workflows to improve yield, scalability, and turnaround time.


• Implement and program automated liquid handling systems, such as Integra Assist Plus and Bravo, for high-throughput laboratory workflows.


• Optimize suspension-based LVV upstream production, including cell expansion, transient transfection, shake flask production, and harvest.


• Apply Design of Experiments (DOE) to optimize plasmid ratios, transfection conditions, reagents, and production parameters.


• Develop downstream purification processes, including depth filtration, microfiltration, tangential flow filtration (TFF), and chromatography.


• Support high-throughput production using automated micro-scale bioreactors, such as Ambr 15 or Ambr 250.


• Perform viral vector characterization and quality control (QC) to evaluate physical and functional attributes.


• Conduct molecular biology assays, including PCR, qPCR, ddPCR, dPCR, and Western blot.


• Analyze experimental data using Excel, GraphPad Prism, and statistical analysis tools.


• Maintain accurate viral vector inventories, electronic laboratory records, and sample traceability.


• Develop and maintain SOPs, technical reports, and technology transfer documentation.


• Present experimental findings and collaborate with multidisciplinary research teams.

Required Qualifications

• Master's degree or PhD in Cell Biology, Molecular Biology, Virology, Biochemistry, Bioengineering, Genetics, or a related discipline.


• 3+ years of laboratory research or industrial experience.


• Hands-on experience with ex vivo CAR T-cell engineering using lentiviral vectors (required).


• Strong proficiency in mammalian cell culture and molecular biology techniques.


• Experience with transient plasmid transfection, PCR, qPCR, ddPCR/dPCR, and Western blot.


• Strong experimental design, data analysis, and scientific presentation skills.


• Excellent organizational skills, attention to detail, and ability to work independently.


• Strong written and verbal communication skills and ability to collaborate with research teams.

Preferred Qualifications

• Experience with lentiviral vector (LVV) or AAV production using adherent or suspension cell cultures.


• Experience with upstream and downstream viral vector process development.


• Knowledge of DOE, statistical modeling, and process optimization.


• Hands-on experience with automated liquid handling platforms, including Integra Assist Plus or Bravo.


• Familiarity with Ambr bioreactors, TFF, chromatography, and viral vector purification.


• Experience operating flow cytometers and performing viral vector characterization.


• Familiarity with electronic laboratory notebooks (ELNs) and BSL-2 laboratory operations.

About BEPC

BEPC Inc., founded in 2007, is a 100% employee-owned company providing top-tier consulting and staffing solutions across industries like technology, engineering, manufacturing, and project management. At BEPC, we are driven by innovation and a commitment to excellence. We take pride in fostering a collaborative and innovative environment where our team members thrive. With competitive benefits, including medical, dental, vision, and life insurance, BEPC is dedicated to supporting our employees' personal and professional growth.