Scientist II

US Tech Solutions; Worcester (MA); In loco; USD 70.000 - 100.000; Tempo pieno

Descrizione del lavoro

A biotechnology firm is seeking a professional for the Cell Line Engineering group to support various applications in the therapeutic development process. The role includes mammalian cell culture, generation and maintenance of cell-lines, FACS analysis, and technology development for cell line engineering. Candidates should have 3-5 years of relevant industry experience. This opportunity provides a chance to expand skills in cell and molecular biology applications and contribute to critical lab initiatives.

Competenze

• 3-5 years of relevant industry experience preferred.

Mansioni

• Support mammalian cell culture work.
• Generate and maintain cell-lines for antibody discovery.
• Conduct FACS analysis and sorting.
• Develop technology for cell line engineering.
• Consolidate cell line assets into digital inventory.

Conoscenze

Mammalian cell culture

Cell line engineering

Flow cytometry

Gene editing by CRISPR/Cas9

Descrizione del lavoro

Overview

This opportunity is in the Cell Line Engineering group within Biotherapeutics Discovery which plays a key role in enabling reagent generation supporting diverse applications throughout the therapeutic development process. Under supervision of a senior scientist in the group, the individual will primarily support mammalian cell culture work, cell line engineering, including KO, KI and OE cell lines development, cellular assays, as well as assist with lab and inventory management and sample digitization initiatives. Opportunities to expand proficiencies in cell and molecular biology applications, such as cell and genome engineering, vector design, technology development, cell-based assays development, receptor density quantification, flow cytometry, cell sorting and cloning, and other cell line development technologies also exist.

Responsibilities

• Mammalian cell culture (immortalized, cancer and primary lines).
• Generation and maintenance of cell-lines for antibody discovery and assay development.
• FACS analysis and sorting.
• Technology development for cell line engineering.
• Consolidation of historical and recent cell line assets into digital inventory solution.
• Expansion and banking of core cell lines to ensure adequate inventories.
• Coordination of cell line inventory stocks across multiple sites, providing a foundation for Cell Bank establishment.
• Mammalian cell culture.
• Cell line engineering.
• Flow cytometry.
• Cell-based assays.
• Gene editing by CRISPR/Cas9.

Education and experience

• 3-5 years of relevant industry experience preferred.

Equal Opportunity Employer: All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.