Scientist I, Discovery Biology

Soufflé Therapeutics; Watertown (MA); On-site; USD 90,000 - 130,000; Full time

Job summary

Soufflé Therapeutics in Watertown, MA seeks a Scientist in Discovery Biology to drive target discovery with in vivo and in vitro data. You will generate, analyze and present data shaping genetic medicine delivery, working with Discovery Biology, Protein, Discovery Technology and In Vivo teams.

You'll design pharmacology and PK/PD studies, isolate primary cells, and help validate new gene targets. This bench-focused role emphasizes meticulous records and clear scientific communication.

Qualifications

• PhD with 0+ years of experience; or MS with 8+ years or BS with 10+ years of relevant industry experience
• Hands-on experience with primary cell isolation from animal and human tissues, and sterile cell culture
• Experience with tissue preservation and processing for downstream analysis
• RNA isolation and downstream analysis (e.g., qPCR, ddPCR)
• Histochemistry and immunostaining (IF), in situ hybridization, microscopy and basic image analysis
• Self-driven, curious, collaborative and detail-oriented, with strong analytical and communication skills

Responsibilities

• Design and analyze in vitro and in vivo pharmacology and PK/PD studies
• Establish and execute methods for cell isolation from tissues
• Establish and validate pre-clinical disease models, both internally and with CRO partners
• Support validation of new gene targets
• Maintain accurate records of experimental procedures and protocols in compliance with electronic laboratory notebook standards

Skills

Cell isolation

Sterile cell culture

RNA isolation

qPCR/ddPCR

Histochemistry

Immunostaining

In situ hybridization

Microscopy

Image analysis

Analytical skills

Communication

Education

PhD

MS with 8+ years

BS with 10+ years

Tools

Laboratory techniques

Job description

Soufflé Therapeutics is a biotech company focusing on the cell-type specific delivery of genetic medicines. It's team of driven scientists is making a mark in delivery of oligonucleotides to extrahepatic tissues. We thrive in a purpose-building lab and office space in Watertown. Come and join us in our quest!

Position Summary:

Soufflé is unlocking a new cell type, and that work requires added hands-on support for a wide range of in vitro and in vivo experiments and analytical techniques. As a Scientist in Discovery Biology, you will drive new target discovery, working in a collaborative, matrixed environment alongside the Discovery Biology, Protein, Discovery Technology and In Vivo teams. This is a new role for someone who wants to spend most of their time at the bench generating, analyzing and presenting data that moves delivery of genetic medicines forward.

Key Responsibilities

• Design and analyze in vitro and in vivo pharmacology and PK/PD studies
• Establish and execute methods for cell isolation from tissues
• Establish and validate pre-clinical disease models, both internally and with CRO partners
• Support validation of new gene targets
• Maintain accurate records of experimental procedures and protocols in compliance with electronic laboratory notebook standards

What Success Looks Like

• In 3 months: You have a clear understanding of your role and the team workflow, contribute to in vitro and in vivo proof-of-concept studies, show strong technical skill in a few key assays, and can design and interpret experiments.
• In 6 months: You are running in vivo studies that validate targeted delivery to cells of interest and enable ligand discovery and validation.
• In 12 months: You independently design and execute studies (proof-of-concept or screening), interpret the data, and present results at internal meetings with minimal guidance.

Qualifications — Required

• PhD with 0+ years of experience; or MS with 8+ years or BS with 10+ years of relevant industry experience
• Hands-on experience with primary cell isolation from animal and human tissues, and sterile cell culture (cell lines and primary human and mouse cells)
• Experience with tissue preservation and processing for downstream analysis
• RNA isolation and downstream analysis (e.g., qPCR, ddPCR)
• Histochemistry and immunostaining (IF), in situ hybridization, microscopy and basic image analysis
• Self-driven, curious, collaborative and detail-oriented, with strong analytical and communication skills

Qualifications — Preferred

• Experience in cardiovascular, lung, metabolic disease research
• Experience with targeted genetic medicines, ideally at an oligonucleotide-focused biotech or pharma company
• Animal tissue collection
• Flow cytometry and ELISA/MSD

Education

At Soufflé Therapeutics, we are intentional about building an equitable culture. How we treat our people is reflective of this focus. We are an Equal Opportunity Employer. We celebrate diversity and are committed to creating an inclusive environment for all employees.

This is an onsite position in Watertown, MA (US)) with flexibility as needed for collaboration and external engagements.