Scientist I - CHO Cell Line Development - # 26-23646

Creative Solutions Services, LLC; South San Francisco (CA); On-site; USD 90,000 - 130,000; Full time

Job summary

US Tech Solutions in South San Francisco, CA is seeking a Scientist I/II to join a collaborative team developing CHO cell lines for GMP applications. The role involves hands-on CHO cell line development and characterization studies, scaling from 96-well plates to shake flasks, and cross-functional collaboration to drive projects forward.

The ideal candidate will have experience with genetic and molecular characterization, as well as instrumentation such as flow cytometers, HPLC, and automated

Responsibilities

• Lead end-to-end CHO cell line development workflows from transfection to screening and scale-up.
• Perform single-cell cloning to establish monoclonal CHO cell lines and bank cell lines.
• Engineer DNA vectors for expressing antibodies and novel protein formats; perform DNA/RNA analysis.
• Operate and maintain instruments including ViCell, Octet, flow cytometers, ambr15, HPLC, and automated imagers.
• Present updates at cross-functional meetings and collaborate with purification, analytical, and formulation teams.
• Maintain meticulous electronic lab notebooks (ELNs) with data integrity.

Skills

CHO cell line development

Flow cytometry

Tools

ViCell

Octet

Flow cytometers

ambr15

HPLC

Automated imagers

Job description

Duration: 12 months

Summary: The Cell Line Development Group in South San Francisco, CA is seeking a Scientist I/II to join a talented and collaborative team developing CHO cell lines for GMP applications. In this laboratory position the candidate will perform hands-on CHO cell line development and characterization studies in a well-supported and dynamic environment to help advance novel biotherapeutics into human clinical studies. The work will encompass molecular, cellular, and bioprocess development studies relating to the generation and characterization of optimized CHO cell lines for production of our cancer pipeline candidates.

Key Responsibilities:

• CHO Cell Line Development & Execution: Lead the end-to-end execution of CHO cell line development workflows for both pipeline and innovation projects. This includes managing post-transfection selective pressures, high-throughput screening, and scaling cultures from 96-well plates to shake flasks.
• Monoclonal Cell Line Generation & Banking: Expertly perform single-cell cloning to generate and establish monoclonal CHO cell lines. Create, expand, and meticulously characterize master and working cell banks.
• Genetic & Molecular Characterization: Engineer and generate complex DNA vector constructs for expressing antibodies and novel protein formats. Perform deep genetic characterization of cell banks by extracting DNA/RNA for analysis via PCR, qPCR/ddPCR, and Next-Generation Sequencing (NGS).
• Advanced Instrumentation & Automation: Skillfully operate and maintain a suite of key laboratory instruments, including ViCell, Octet, flow cytometers, ambr15, HPLC, and automated imagers.
• Cross-Functional Collaboration & Communication: Present scientific updates at cross-functional meetings, collaborating closely with colleagues in purification, analytical, and formulation development to drive projects forward and meet aggressive timelines.
• Data Integrity & Documentation: Maintain meticulous, contemporaneous records of all experimental designs, data, and analysis in electronic lab notebooks (ELNs), ensuring compliance withClient standards for data integrity and good documentation practices.

Top 3-5 skills needed

1. CHO cell line development
2. Flow cytometry

About US Tech Solutions:

US Tech Solutions is a global staff augmentation firm providing a wide range of talent on-demand and total workforce solutions. To know more about US Tech Solutions, please visit www.ustechsolutions.com.

Equal Employment Opportunity (EEO):

US Tech Solutions is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, colour, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

AI Statement:

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