Scientist

Mindlance

Framingham (MA)

On-site

USD 66,124 - 71,635

Full time

14 days+

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Job summary

A leading biotechnology firm is looking for a Scientist I to join their Cell Line Development Department in Framingham, MA. This role focuses on optimizing CHO cell line development platforms through advanced molecular biology techniques. The candidate should have a PhD with hands-on experience in molecular cloning and automated workflows. Responsibilities include designing expression vectors, developing high-throughput processes, and characterizing cell lines. This is a contract position with a duration of 12+ months and a competitive pay rate of $48-$52 per hour.

Qualifications

PhD in Molecular Biology, Cell Biology, Biochemistry or related field.
Experience with molecular cloning techniques including Gibson assembly and Golden Gate.
Proficiency in automated molecular biology workflows.

Responsibilities

Design expression vectors using advanced molecular cloning techniques.
Develop automated workflows for high-throughput vector construction.
Perform molecular characterization of cell lines and constructs.

Skills

Molecular cloning techniques

DNA construct design

Automated workflows

Attention to detail

Team collaboration

Education

PhD in Molecular Biology or related discipline

Tools

Gibson assembly

Golden Gate cloning

ddPCR

Sanger sequencing

Hamilton liquid handling platforms

Job description

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Base pay range

$48.00/hr - $52.00/hr

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Hiring Pharmaceutical, Medical Devices, Healthcare, Clinical, Biotech & LifeSciences professionals

Job Title: Scientist I

Location: Framingham, MA

Duration: 12+ months with possible extension

The Cell Line Development Department at is seeking a highly motivated Molecular Biology Scientist to join a high-performing, collaborative team focused on optimizing CHO cell line development platforms through advanced molecular biology techniques and automation. Responsibilities will focus on improving process workflows, expression system components, and CLD methods used to generate recombinant CHO cell lines for production of protein biologics. This will include the design and generation of next generation vector topologies utilizing modern cloning strategies such as Gibson assembly and Golden Gate cloning. The ideal candidate will possess expertise in high-throughput molecular biology workflows and automated vector construction pipelines. Work will directly support development projects in pipeline and will include frequent cross-functional interactions with colleagues in Sanofi's global CMC sites (US, France).

Key Responsibilities

Design and construct expression vectors using advanced molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction enzyme-based cloning, and site-directed mutagenesis
Develop, optimize, and implement automated workflows for high-throughput vector construction and DNA assembly on liquid handling platforms
Generate, validate, and bank vector constructs for expression of candidate biotherapeutic molecules in mammalian cell systems
Perform molecular characterization of cell lines and constructs using techniques such as ddPCR, Sanger sequencing, and restriction mapping
Support NGS library preparation for genomic and transcriptomic analysis of CHO cell lines
Optimize and troubleshoot molecular biology protocols to improve efficiency and reproducibility
Prepare technical reports and presentations to communicate progress and data

Basic Qualifications

PhD (0-3 years) in Molecular Biology, Cell Biology, Biochemistry, Bioengineering, or related discipline
Extensive hands-on experience with molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction cloning, and PCR-based methods
Demonstrated expertise in DNA construct design, vector engineering, and plasmid preparation
Experience with genetic characterization methods such as ddPCR, qPCR, gel electrophoresis, and DNA sequencing analysis
Proficiency in designing and optimizing molecular biology workflows for automation on liquid handling platforms (Hamilton, Tecan, or similar)
Strong technical background in molecular biology with excellent attention to detail and troubleshooting skills
Self-motivated with excellent organization, time-management, and communication skills
Maintain detailed electronic laboratory notebooks and documentation of all experimental procedures
Demonstrated ability to work as member of a team and adhere to timelines

Preferred Qualifications

Mammalian cell culture experience in a pharmaceutical or biotechnology setting, particularly with CHO cells
Experience with high-throughput screening and clone selection strategies
Familiarity with bioinformatic tools for DNA sequence analysis, primer design, and in silico cloning (Snapgene, Geneious, Benchling, Primer3 or similar)
Knowledge of CHO cell line development processes and recombinant protein expression
Experience using Oxford Nanopore Technologies in NGS workflows
Basic programming skills in R or Python for data analysis and visualization
Experience with Bash/command line scripting for bioinformatics workflows and data processing
Experience with automated colony picking and clone tracking systems
Flexibility to adjust to a fast-paced, goal-oriented environment and willingness to expand and broaden skillset

As a healthcare company and a vaccine manufacturer, has an important responsibility to protect individual and public health. All US based roles require individuals to be fully vaccinated against COVID-19 as part of your job responsibilities.

EEO:

Mindlance is an Equal Opportunity Employer and does not discriminate in employment on the basis of – Minority/Gender/Disability/Religion/LGBTQI/Age/Veterans.

Seniority level