Scientist I

Tailored Management; Cambridge (MA); Sur place; USD 62 000 - 70 000; Plein temps

Résumé du poste

Tailored Management seeks a Scientist for the Neurology Research Unit's ALS group in Cambridge, MA. The role focuses on exploratory discovery of molecular and cellular mechanisms underlying ALS and related neurodegenerative diseases, using genomics, proteomics, and cellular assays.

You will design and perform a range of experiments, generate reproducible data, and work with a multidisciplinary team to advance therapeutic targets. The position is onsite for a 12-month term.

Qualifications

• Strong foundation in molecular and cellular biology with genomics/proteomics understanding.
• Hands-on molecular biology techniques: qPCR, ddPCR, gel electrophoresis, Western blotting.
• Experience with mammalian cell culture, transfection or viral transduction.
• Experience in protein analysis, immunostaining, fluorescence microscopy or cellular assays.
• Analytical, troubleshooting, and problem-solving skills.
• Able to work independently and collaboratively in a multidisciplinary team.

Responsabilités

• Design, perform, and analyze molecular biology experiments including qPCR/ddPCR.
• Conduct protein-based assays and proteomic studies using HTRF/AlphaLISA/MSD platforms.
• Perform mammalian cell culture, transfection, and viral transduction.
• Support target validation through immunostaining and fluorescence microscopy.
• Co-localization, morphological and phenotypic assessments for ALS targets.
• Assist with experimental design, assay development, optimization and troubleshooting.
• Generate and document reproducible data, create figures and presentations.
• Maintain accurate records and ensure safety, quality and data integrity.

Connaissances

Molecular biology

Cell culture

qPCR

ddPCR

Formation

Master's or PhD in Molecular Biology

Outils

qPCR

ddPCR

Gel electrophoresis

Western blotting

Description du poste

Scientist – Neurology Research (ALS Research Group)

Location: Cambridge, MA (Onsite)

Pay Rate: $45.00–$51.00/hour

Contract: 12 Months (January 2027 – December 2027)

About the Role

We are seeking a highly motivated Scientist to join the Neurology Research Unit's ALS Research Group in Cambridge, MA. This individual will support exploratory discovery research focused on understanding the molecular and cellular mechanisms underlying Amyotrophic Lateral Sclerosis (ALS) and related neurodegenerative diseases.

The successful candidate will contribute to early-stage research involving target identification and validation through molecular biology, genomics, proteomics, and cellular assays. This role offers an opportunity to collaborate with experienced scientists, generate high-quality experimental data, and support research initiatives aimed at advancing the understanding of ALS biology and potential therapeutic targets.

Key Responsibilities

• Design, perform, and analyze molecular biology experiments, including quantitative PCR (qPCR), droplet digital PCR (ddPCR), gel electrophoresis, and Western blotting to evaluate gene and protein expression.
• Conduct protein-based assays and proteomic studies, including immunoprecipitation (IP) and protein quantification using HTRF, AlphaLISA, and MSD platforms.
• Perform routine mammalian cell culture, plasmid transfection, and viral transduction using human iPSC-derived neurons and established cell lines.
• Support target validation studies through immunostaining, fluorescence microscopy, and quantitative image analysis.
• Conduct co-localization, morphological, and phenotypic assessments to characterize candidate therapeutic targets relevant to ALS and other neurodegenerative diseases.
• Assist with experimental design, assay development, optimization, validation, and troubleshooting to support ongoing discovery research projects.
• Generate, analyze, and document reproducible experimental data, including preparing data summaries, figures, and presentations for internal scientific discussions.
• Maintain accurate laboratory records and ensure all research activities comply with established laboratory safety, quality, and data integrity standards.

Required Qualifications

• Strong foundation in molecular and cellular biology, with a solid understanding of genomics and proteomics.
• Hands-on experience with molecular biology techniques, particularly qPCR, ddPCR, gel electrophoresis, and Western blotting.
• Experience with mammalian cell culture, including plasmid transfection and/or viral transduction.
• Experience performing protein analysis, immunostaining, fluorescence microscopy, or other cellular characterization assays.
• Strong analytical, troubleshooting, and problem-solving skills.
• Ability to independently execute laboratory experiments while collaborating effectively with a multidisciplinary research team.
• Excellent experimental documentation skills and attention to detail.

Preferred Qualifications

• Experience working with human iPSC-derived neurons or neuronal cell models.
• Background in ALS, neuroscience, or other neurodegenerative disease research.
• Experience with immunoprecipitation and protein quantification platforms such as HTRF, AlphaLISA, and MSD.
• Experience with quantitative image analysis, co-localization studies, and cellular phenotypic assessments.
• Previous involvement in assay development, optimization, and target validation studies.

Education

• Master's or PhD in Molecular Biology, Biochemistry, Neuroscience, Cell Biology, or a related field.
• PhD-level research training or equivalent research experience is highly desirable.