Scientist Bioscience

The Fountain Group; Cambridge (MA); On-site; USD 100,000 - 110,000; Full time

Job summary

The Fountain Group in Cambridge, MA, seeks a Research Scientist specialized in Chemical Biology & Proteomics to apply MS-based proteomics in drug discovery and mechanism-of-action studies. You will design and execute proteomic assays, perform affinity enrichment and proximity biotinylation workflows, and analyze data to investigate protein interactions.

The role involves mammalian cell culture, protein conjugation/purification, UV-Vis characterization, and target validation using cell-based

Qualifications

• Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or related field with 2+ years hands-on proteomics experience.
• Master's with 1+ year industry proteomics experience and 3–5 years hands-on lab experience.

Responsibilities

• Apply mass spectrometry-based proteomics in drug discovery and mechanism-of-action studies.
• Design and execute proteomic assays, affinity enrichment, and proximity biotinylation workflows.

Skills

Proteomics

Cell culture

Affinity enrichment

Data analysis

Experiment design

Education

Ph.D. or equivalent

Master's with industry proteomics

Tools

LC-MS/MS

Spectronaut

Proteome Discoverer

MaxQuant

Skyline/Xcalibur

Job description

Job Title: Research Scientist - Chemical Biology & Proteomics
Location: Cambridge, MA (100% Onsite)
Pay Rate: $50/hour
Duration: 12 Months
Schedule: Monday-Friday, 8:00 AM-4:00 PM

Job Description

Join the Chemical Biology and Proteomics team to apply mass spectrometry-based proteomics in drug discovery and mechanism-of-action studies. Design and execute proteomic assays, perform affinity enrichment and proximity biotinylation workflows, and analyze experimental data to investigate protein interactions and disease biology. Perform mammalian cell culture, protein conjugation, purification, and UV-Vis spectroscopy characterization. Conduct target validation using cell-based assays and maintain accurate electronic laboratory records.

Education

• Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or a related discipline, with at least 2 years of hands-on proteomics experience, including graduate or postdoctoral research; OR

• Master's degree with at least 1 year of industry proteomics experience and 3-5 years of hands-on laboratory experience.

Required Skills

• Hands-on proteomics experience, including proteomic sample preparation and mass spectrometry-based analysis.

• Experience with mammalian cell culture.

• Experience with affinity enrichment workflows and protein characterization.

• Experience using cell-based and proteomic assays to investigate small-molecule mechanisms of action.

• Data analysis and interpretation using established bioinformatics pipelines.

• Ability to independently design and execute experiments and interpret scientific results.

Preferred Skills

• Experience developing or applying proximity ligation assays (PLA) or other protein-protein interaction assays.

• Experience with proximity biotinylation and data-independent acquisition (DIA) mass spectrometry.

• Hands-on experience with LC-MS/MS platforms and semi-automated instrumentation.

• Familiarity with Spectronaut, Proteome Discoverer, MaxQuant, Skyline, or Xcalibur.

• Experience with confocal microscopy and protein conjugation techniques.