Research Technician, Neuroscience

Tufts University; Boston (MA); On-site; USD 28,000 - 39,000; Full time

Job summary

Tufts University's Department of Neuroscience is seeking a Laboratory Technician to support cutting-edge research into Alzheimer's disease mechanisms. Under supervision, you will culture human iPSCs, differentiate them into neurons and glia, and help characterize cultures using biochemistry, microscopy, and molecular biology techniques.

Responsibilities include maintaining a mouse colony, genotyping, collecting brain samples, keeping equipment and supplies in order, and documenting data.

Qualifications

• Bachelor's degree in science or related field.
• At least one year of related laboratory experience.
• Experience with stem cell culture and genome editing is a plus.
• Experience with mouse colonies and brain tissue sampling is preferred.

Responsibilities

• Culture human iPSCs and differentiate them into neurons and glia.
• Maintain mouse colony, perform genotyping, and collect brain samples.
• Maintain laboratory equipment and inventory.
• Conduct experiments (biochemistry, microscopy, molecular biology) and document data under supervision.
• Work beyond regular hours as experiments demand; follow BSL2 safety protocols.

Skills

iPSC culture

Genome editing

Microscopy

Western blotting

RNA/DNA extraction

PCR/qPCR

Education

Bachelor's degree in science

Job description

Overview

The Department of Neuroscience is a basic science department that focuses on cutting-edge research in the areas of neurological disease. Dr. Giuseppina Tesco is supported by funding from the federal government and private foundations to study the molecular mechanism of Alzheimer's disease.

What You'll Do

Under close supervision of Principal Investigator, conducts a variety of routine experiments following established procedures and protocols. There are two primary functions of this position: 1) Culturing human iPSCs and differentiating them in neurons and glia cells, 2) Characterization of cultures with biochemistry, microscopy and molecular biology (Protein extraction Western blotting, ELISA, immunostaining and microscopy, RNA and DNA extraction, PCR and qPCR, genome editing). Having prior experiences in stem cell culture and genome editing is a plus. Maintaining mouse colony.

• Culturing human iPSCs and differentiating them in neurons and glia cells
• Maintaining mouse colony, genotyping and collection of brain samples.
• Maintains laboratory equipment and orders/keeps inventory of supplies.
• Perform experiments (e.e biochemistry, microscopy and molecular biology ), and documenting data under researcher guidance.
• Occasional need to work beyond regular work hours, holidays, or weekends as experiments demand. Work with protective attire and in BSL2 safety cabinets for animal infections and multi-drug-resistant bacteria is required.

What We're Looking For

Basic Requirements:
Knowledge and experience typically acquired by:

• Bachelor's degree in science
• At least one year of related laboratory experience

Preferred Qualifications:

• Advanced knowledge and experience with laboratory techniques and procedures
• Knowledge of basic statistics

Pay Range

Minimum $20.50, Midpoint $24.40, Maximum $28.30



Salary is based on related experience, expertise, and internal equity; generally, new hires can expect pay between the minimum and midpoint of the range.