Technical Research Assistant II

2200 The Brigham and Women's Hospital, Inc.; Boston (MA); On-site; USD 33,000 - 54,000; Full time

Job summary

The Brigham and Women's Hospital, Inc. is seeking a recent college graduate for an on-site basic research role in a dedicated lab building.

You will work independently under the Principal Investigator, performing routine experiments and data analyses to study human protein-DNA interactions and transcriptional regulation. Responsibilities include following established protocols, performing sequencing prep, literature searches, and contributing to grant-ready figures and manuscripts.

Qualifications

• Fluent command of English and strong written/oral communication.
• Strong analytical and organizational skills. Computer literacy required.
• Ability to work in a Linux environment with attention to detail.

Responsibilities

• Perform routine laboratory procedures following established protocols.
• Prepare solutions, media, cultures, and sequencing samples as assigned.
• Analyze data and prepare figures; present findings to supervisor weekly.
• Maintain records, equipment, and clean working environments.

Skills

Linux environment

Computer literacy

English communication

Attention to detail

Analytical thinking

Job description

Site: The Brigham and Women's Hospital, Inc. Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Job Summary

This position is for primarily on-site, basic research in a research lab located in a dedicated research building (not a clinical hospital setting). Working independently, under the general supervision of the Principal Investigator, will conduct a variety of routine experiments where methodologies are clearly established and will determine whether results warrant repeating or modification. Calculates, transcribes, and analyzes data using basic statistics. May perform literature searches under direction of supervisor. This position will focus on studies of human protein-DNA interactions and DNA regulatory elements by a combination of biochemical and cell culture based approaches. These data will aid in understanding mechanisms of transcriptional gene regulation and the impact of coding and noncoding genetic variation. This position provides an excellent opportunity to be involved in cutting-edge research in gene regulation and genomics, and offers a stimulating, academic environment, and the opportunity to work in a team setting involving experimental and computational scientists. This position also offers the opportunity to gain valuable written and oral communication skills, and the possibility for co-authorship on publications. This position is ideal for a recent college graduate seeking to gain additional laboratory research experience.

Qualifications

Working independently, under general supervision, performs routine laboratory procedures following established protocols. These procedures may include: preparation of solutions and media, growth of bacterial cultures, DNA mini- and midi-preps, submitting DNA samples for Sanger and Illumina DNA sequencing, preparation of E. coli glycerol stocks, mammalian cell culture, CRISPR/Cas genome editing, Gateway recombinational cloning, preparation of Illumina DNA sequencing libraries, restriction enzyme based or Gibson assembly DNA cloning, expression and purification of recombinant proteins, DNA and protein gel electrophoresis, electrophoretic mobility shift assays (EMSA), PCR, Western blots, Coomassie or other staining of protein gels, in vitro transcription and translation, quantitative real-time PCR, RNA purification, preparation of Illumina DNA sequencing libraries.

Other duties may include: protein binding microarray (PBM) experiments and data analysis; other biochemical assays of protein-DNA binding activities; mammalian cell culture; lentiviral transduction of mammalian cells; chromatin immunoprecipitation (ChIP).

Working independently, under general supervision or with instruction as needed, analyzes Sanger sequencing results, analyzes Illumina sequencing reads, analyzes quantitative real-time PCR data, performs DNA sequence motif analysis, protein sequence analysis and other computational sequence analyses, and prepares figures using R and other statistical analysis tools.

Maintains complete daily records of methodologies and test results in laboratory notebook. May present results in lab meeting presentations. May prepare figures or sections of text for grant applications or manuscripts. Regularly reports findings to supervisor on a weekly basis. Identifies unusual findings and reports such findings to supervisor. Maintains equipment and supplies in a neat and orderly manner. Maintains chemical hood and tissue culture hood in a neat and orderly manner. Maintains chemical weigh bench and gel running bench in a clean & orderly state, including re-applying bench liners. Maintains the sinks in a clean & orderly state. Maintains established department policies, procedures, objectives, quality assurance, safety, environmental and infection control. Performs other duties and responsibilities as directed including providing technical support for postdoctoral associates, graduate students, and senior technical research staff working in the laboratory under the Principal Investigator’s supervision. Submits and/or places orders for supplies, chemicals, and other reagents.

Competencies

• Must have a sound understanding of laboratory policies and practical experience in laboratory techniques and procedures.
• Sound analytical and organizational skills.
• High degree of computer literacy.
• Must be able to work in a Linux environment.
• Must have good manual dexterity.
• Must have a flexible work schedule.
• Must demonstrate careful attention to detail.
• Ability to identify potential problems and trouble-shoot solutions.
• Must be able to maintain a neat and orderly working environment.
• Fluent command of spoken and written English is absolutely required.
• Good interpersonal and communication skills.
• Ability to demonstrate professionalism.

Additional Job Details

• Remote Type: Onsite
• Work Location: 77 Avenue Louis Pasteur
• Scheduled Weekly Hours: 40
• Employee Type: Regular
• Work Shift: Day (United States of America)
• Pay Range: $24.28 - $39.43/Hourly
• Grade: 6

EEO Statement

2200 The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.

Mass General Brigham Competency Framework

At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline. Combat disease. Hold a hand. Help people. Impact the world. Mass General Brigham is a passionate, welcoming community where minds meet caring hearts. Come be a part of the world’s most powerful force in medicine, where every role is important in changing lives. Are you ready? Our history includes New England hospitals founded over 200 years ago, some of the first and most prestigious hospitals in the world. Built on the legacy of two leading academic medical centers, we’re more than a system—we’re leaders in the practice of medicine. Mass General Brigham is committed to serving the community. We are dedicated to enhancing patient care, teaching and research, and taking a leadership role as an integrated health care system. We recognize that increasing value and continuously improving quality are essential to maintaining excellence.