Research Technician I

Children's National Medical Center; Washington (District of Columbia); On-site; USD 42,000 - 72,000; Full time

Job summary

An established industry player in pediatric healthcare is seeking a Research Technician I to join a dynamic lab focused on cancer biology. This role involves conducting vital research on microRNAs and extracellular vesicles, performing specialized laboratory tests, and collaborating with a dedicated team. The ideal candidate will possess strong skills in mammalian cell culture and PCR techniques, with a passion for scientific inquiry. If you're eager to contribute to groundbreaking research and make a difference in children's health, this opportunity is perfect for you. Join a forward-thinking organization committed to innovation and excellence in research.

Qualifications

• Bachelor's degree in a related field preferred.
• 1-2 years of related experience desired.

Responsibilities

• Conduct routine and specialized laboratory tests under direction.
• Assist with planning research protocols and procedures.

Skills

Mammalian cell culture

PCR

Western blotting

FACS

In vivo work with murine models

Quantitative Real-Time PCR

Immuno-precipitation

Mycoplasma testing

Cell transfection

Data analysis

Education

Bachelor's Degree in Basic Science

Tools

Bioluminescence imaging devices

Basic lab equipment

Job description

Job Description - Research Technician I (2400034F)

POSITION SUMMARY:

Dr. Fabbri’s lab at Children’s National is recruiting a Research Technician I. The RT-I conducts research under Dr. Fabbri’s direction. Dr. Fabbri’s lab studies the role of microRNAs and other non-coding RNAs in cancer biology and the role of extracellular vesicles in cell-to-cell communication. The RT-I performs a variety of routine and specialized laboratory tests, specimen preparations and experimental procedures for the purpose of research as directed.

QUALIFICATIONS:

Required experience in the following techniques:

• Mammalian cell culture/propagation/freezing
• Mycoplasma testing of cultured cells
• Peripheral Blood Mononuclear Cell isolation from peripheral blood
• Mammalian cell transfection of microRNAs, siRNAs
• Familiarity with viral systems to perform stable gene transduction to mammalian cells
• PCR and all aspects of cloning (bacteria propagation of plasmids, mini/maxi preps etc…)
• Quantitative Real-Time PCR
• Western blotting and ELISA
• Immuno-precipitation of RNA/protein (or ability to follow a provided protocol for IP)
• FACS
• In vivo work with murine models: subcutaneous and orthotopic injections of cells, ability to perform intravenous/intraperitoneal/subcutaneous injections in mice, ability to perform in vivo imaging with bioluminescence devices (such as IVIS or similar), ability to accurately measure tumor growth with a caliper

Desired experience in:

• Isolation and characterization of exosomes and other extracellular vesicles
• Ability to generate nanoparticles for specific delivery of therapeutic cargo to target cells

Minimum Education:
Bachelor's Degree with a major in basic science or related field desired. (Preferred)

Minimum Work Experience:
At least 1-2 years of related experience desired. No supervisory experience required. (Preferred)

Required Skills/Knowledge:
Strong mathematical skills and an inquisitive attitude. Basic knowledge of lab safety and use of basic lab equipment desired.

Functional Accountabilities:

Responsible Conduct of Research:

• Consistently demonstrates adherence to the standards for the responsible conduct of research.
• Plans, conducts and manages research projects within federal and institutional regulations and policies under the direction of the principal investigator.
• Uses research funds and resources appropriately.
• Maintains confidentiality of data as required.
• Meets all annual job-related training and compliance requirements.

Laboratory Work:

• Performs routine tests under the direction of principal investigator or supervisor.
• Records and checks results for completeness and accuracy.
• Conducts literature search as requested.
• Performs tasks that may require familiarity with fundamental techniques and equipment.
• Compiles information from lab notebooks and journals.
• Attends courses, seminars and lab meetings to expand knowledge of research and laboratory technique.

Project Planning:

• Assists with planning specific research protocols and procedures.
• Coordinates schedules of tests and procedures.
• Assists staff in developing and troubleshooting new techniques and ideas.

Recordkeeping:

• Maintains a log of work performed.
• Prepares charts, graphs and results of studies; compiles and helps analyze data.
• Maintains all required records, reports, statistics, etc.; prepares summary reports as requested.
• Maintains and updates department policies regarding procedures, objectives, safety, etc.
• Anticipate and responds to customer needs; follows up until needs are met.

Teamwork/Communication:

• Demonstrate collaborative and respectful behavior.
• Partner with all team members to achieve goals.
• Receptive to others’ ideas and opinions.

Performance Improvement/Problem-solving:

• Contribute to a positive work environment.
• Demonstrate flexibility and willingness to change.
• Identify opportunities to improve clinical and administrative processes.
• Make appropriate decisions, using sound judgment.
• Use resources efficiently.
• Search for less costly ways of doing things.

Safety:

• Speak up when team members appear to exhibit unsafe behavior or performance.
• Continuously validate and verify information needed for decision making or documentation.
• Stop in the face of uncertainty and takes time to resolve the situation.
• Demonstrate accurate, clear and timely verbal and written communication.
• Actively promote safety for patients, families, visitors and co-workers.
• Attend carefully to important details - practicing Stop, Think, Act and Review in order to self-check behavior and performance.

Primary Location: District of Columbia-Washington

Work Locations: CN Hospital (Main Campus) 111 Michigan Avenue NW Washington 20010

Job: Research

Organization: Ctr Cancer & Immunology Rsrch

Position Status: R (Regular) - FT - Full-Time

Shift: Day

Work Schedule: 9:00-5:30 PM

Full-Time Salary Range: 42702.4 - 71156.8

Childrens National Hospital is an equal opportunity employer that evaluates qualified applicants without regard to race, color, national origin, religion, sex, age, marital status, disability, veteran status, sexual orientation, gender, identity, or other characteristics protected by law.

Please note that it is the policy of Children's National Hospital to ensure a “drug-free” work environment: a workplace free from the illegal use, possession or distribution of controlled substances (as defined in the Controlled Substances Act), or the misuse of legal substances, by all staff (management, employees and contractors).