Research Associate I: Neurodegeneration & iPSC Imaging

Gladstone Companies, Inc.; San Francisco (CA); On-site; USD 39,000 - 45,000; Full time

Benefits offered by this job

Competitive salary

Excellent benefits

Retirement plan

Commuter benefits

Free shuttle transportation

Job summary

Gladstone Institutes in San Francisco seeks a Research Associate I to join a team studying age-dependent neurodegeneration and neuroinflammation, using iPSC-derived neurons and glia, high-throughput imaging, and ML approaches. You will contribute to mechanistic disease biology and identify therapeutic targets in ALS, FTD, AD, PD, and HD.

The role involves culture, imaging, molecular biology, data analysis, and collaboration with industry partners.

Qualifications

• B.S. or M.S. in Biology, Biotechnology, or related field or equivalent combination of education and experience.
• 0-2 years of research experience including basic biology skills and excellent cell culture sterile technique.
• Experience with cell culture, microscopy, and cellular and molecular biology techniques.

Responsibilities

• Maintain and differentiate human patient iPSCs to neurons, microglia, astrocytes, and organoids, and carry out cell transfections, nucleofections, or viral transductions and perform cell-based assays using imaging and other approaches.
• Perform automated and manual cell-based imaging assays with novel biosensors for cell survival, neuroinflammation, autophagy, lysosome function, protein trafficking, and other cell-based functions.
• Perform standard assay techniques such as ELISA, western blotting, immunocytochemistry, and immunofluorescence and confocal microscopy and spectral imaging.
• Perform standard molecular biology techniques such as DNA preparation and restriction enzyme digestions, DNA cloning and plasmid construction, gel electrophoresis, and qPCR.
• Perform manual curation and statistical analysis of imaging data using basic computational skills (R-analysis, Galaxy pipeline workflow construction, ImageJ/Fiji) to generate high quality datasets for machine learning or publication.
• Review data, prepare figures and reports, and communicate results to scientists and the general public in team and departmental meetings and conferences.
• Participate in collaborative work with multi-disciplinary teams
• Maintain and complete documentation of all experimental procedures in written and electronic lab notebooks.

Skills

Cell culture

Microscopy

Molecular biology

Data analysis

Communication

Team collaboration

Experiment troubleshooting

Education

B.S. or M.S. in Biology or related field

Tools

ImageJ/Fiji

R

Galaxy pipeline

Nextflow

Python

qPCR

Job description

Gladstone Institutes in San Francisco seeks a Research Associate I to join a team studying age-dependent neurodegeneration and neuroinflammation, using iPSC-derived neurons and glia, high-throughput imaging, and ML approaches. You will contribute to mechanistic disease biology and identify therapeutic targets in ALS, FTD, AD, PD, and HD.

The role involves culture, imaging, molecular biology, data analysis, and collaboration with industry partners.