Research Associate - Biocatalysis

Cascade Bio; Denver (CO); On-site; USD 65,000 - 90,000; Full time

Job summary

Cascade Biocatalysts seeks a Research Associate to join our enzyme team in designing, executing, and advancing internal and customer-driven enzyme projects. You will assist with expression, purification, and characterization of enzyme variants, supporting assay development and recombinant protein tasks.

As an early hire in a startup, you will collaborate across biochemists, chemical engineers, and chemists, contribute to SOPs, data analysis, and documentation, and help scale our Body Armor for

Qualifications

• Bachelor’s degree in biochemistry, molecular biology, biology, chemistry, chemical engineering, or related field with 1–2 years of relevant work or research experience.
• Experience with enzyme assays, bioconjugation, PCR, SDS-PAGE, or cloning.
• Strong analytical, detail-oriented, and collaborative teamwork skills.

Responsibilities

• Develop and execute enzyme activity assays to support screening and characterization.
• Perform molecular cloning and plasmid construction for variant libraries.
• Express and purify recombinant enzymes and assess purity.
• Collaborate across biochemists, chemical engineers, and chemists on experiments.
• Maintain meticulous records and analyze data with appropriate software.

Skills

Enzyme assays

Bioconjugation

PCR

SDS-PAGE

Protein expression

Cloning

Data analysis

Documentation

Education

Bachelor's degree in Biochemistry or related field

Tools

Cell-free protein synthesis

Job description

Job Title: Research Associate – Biocatalysis

Type:

Full-time

Primary responsibility:

Assist our enzyme team in designing, executing, and advancing internal and customer-driven enzyme projects

Job Description:

Cascade Biocatalysts is seeking a dynamic and motivated Research Associate to join our growing team. Reporting directly to one of our Scientists, the Research Associate will play a pivotal role in expanding, testing, and characterizing Cascade’s enzyme portfolio. You will also utilize Cascade’s novel Body Armor for Enzyme™ immobilization technology to improve the commercial viability of these enzymes. This role will support enzyme assay development and execution, molecular cloning, recombinant protein expression, bioconjugation, and our enzyme engineering pipeline. Your role will also support broader R&D and screening efforts as the team scales.

Responsibilities:

• Enzyme Assay Development & Execution: Execute and help troubleshoot enzyme activity assays to support screening and characterization of engineered enzyme variants.
• Molecular Biology & Cloning: Perform molecular cloning, plasmid construction, and related DNA handling to generate constructs for enzyme variant libraries.
• Recombinant Protein Expression & Purification: Express and purify recombinant enzymes (including via cell-free protein synthesis), and assess purity and identity using techniques such as SDS-PAGE.
• Bioconjugation: Support bioconjugation experiments utilizing Cascade’s Body Armor for Enzyme™ immobilization technology
• Startup Environment: As an early hire at a startup, you must be able to adapt to rapidly evolving project needs and fast paced project timelines, deadlines and contribute to cross-functional tasks as required.
• Collaborative teamwork: Collaborate within a cross-functional team, including biochemists, chemical engineers, and chemists, to design experiments, troubleshoot issues, and contribute to project planning and execution.
• Data collection, analysis, and record keeping: Maintain meticulous, well-organized records of experimental procedures, observations, and results. Organize and analyze data using appropriate statistical methods and software.
• Documentation & Communication: Contribute to the development of standard operating procedures (SOPs) for assay development, cloning, and protein expression workflows. Prepare clear reports, summaries, and presentations to effectively communicate research findings and progress to the team.
• Laboratory maintenance: Maintain cleanliness and organization of the laboratory space. Assist in routine maintenance and calibration of laboratory equipment.

Qualifications:

• Hands-on lab experience: Experience in a research laboratory environment, with exposure to biochemistry and/or molecular biology techniques such as enzyme assays, bioconjugation, PCR, SDS-PAGE, assay development, recombinant protein expression, or cloning preferred.
• Bachelor's degree in biochemistry, molecular biology, biology, chemistry, chemical engineering, or a related field and 1-2 years of relevant work or research experience in protein biochemistry, enzymology, molecular biology, or related disciplines.
• Strong analytical and problem-solving skills.
• Excellent attention to detail and organizational abilities.
• Effective communication skills and the ability to work collaboratively within a team.
• Flexibility and adaptability to meet the challenges of a startup environment.
• Eagerness to learn and willingness to take on diverse responsibilities.
• Knowledge of laboratory safety protocols and commitment to maintaining a safe working environment.

Cascade Biocatalysts is an equal opportunity employer.

We welcome and encourage diversity in the workplace and strongly encourage candidates from all backgrounds to apply.

About Cascade Biocatalysts:

Cascade Biocatalysts is empowering enzymes to change how molecules are made. We are commercializing our Body Armor for EnzymesTM technology to make enzymes rugged and reliable for cost effective biomanufacturing and, ultimately, a sustainable, more capable 21st century chemical economy. You will play a pivotal role in developing and improving the enzymes that power our products. Your expertise will contribute to ensuring the quality, reliability, and performance of our enzymes as we commercialize innovative biocatalytic technology. This is an exciting opportunity to make a significant impact within a dynamic and growing organization.

Join us in our mission to revolutionize sustainable chemical manufacturing through the power of enzymes and polymers. Your contributions can help create a greener and more sustainable future for all.