Research Assistant III

Georgetown University; Vinalhaven (ME); Presencial; USD 25.000 - 37.000; Jornada completa

Descripción de la vacante

Georgetown University Medical Center, Department of Microbiology and Immunology, is seeking a Research Assistant III (Laboratory Technician) to support grant-funded studies in the woodchuck model. The role involves coordinating sample processing and performing assays to determine viral titers and immune responses in blood, liver, and other tissues.

The candidate will train and supervise undergraduates and graduates, handle woodchuck samples, and ship materials to collaborating labs.

Formación

• Bachelor’s degree or higher with several years of lab experience.
• Experience with viral and host assays for HBV or similar viruses preferred.
• Ability to follow standardized laboratory protocols and adapt to woodchuck-specific assays.
• May require university certification for handling radioisotopes (32P).

Responsabilidades

• Process woodchuck samples (blood, serum, liver, tissues).
• Perform viral and immune assays, including ELISAs and real-time PCR.
• Train and supervise Undergraduate and Graduate students.
• Ship samples to diagnostic or collaborating laboratories.

Conocimientos

Viral assays

Host assays

ELISA

qPCR

FACS

Sample processing

Data organization

Educación

Bachelor's degree or higher

Herramientas

Radioisotope handling (32P)

Descripción del empleo

Georgetown University comprises two unique campuses in the nation’s capital. With the Hilltop Campus located in the heart of the historic Georgetown neighborhood, and the Capitol Campus, just minutes from the U.S. Capitol and U.S. Supreme Court, Georgetown University offers rigorous academic programs, a global perspective, and unparalleled opportunities to engage with Washington, D.C. Our community is a close-knit group of remarkable individuals driven by intellectual inquiry, a commitment to social justice, and a shared dedication to making a difference in the world.

Requirements

Job Overview

We are looking to hire a Research Assistant III (Laboratory Technician) within the Department of Microbiology and Immunology, Georgetown University Medical Center, School of Medicine – Georgetown University.

This position is critical to the successful implementation of grant-funded studies in the woodchuck animal model, and as such, it is expected that the applicant has prior laboratory experience and is familiar with routine assays and methods, such as ELISA and quantitative PCR. Together with the PI, he/she coordinates and manages woodchuck-specific sample processing and then performs all assays/methods independently, including the determinations of viral titers and immune responses in blood, liver and other tissues of woodchucks. The individual is responsible for the handling and processing of stat and archived samples. He/she eventually trains and supervises Undergraduate and Graduate students during internships in relevant sample processing and laboratory assays. This position also involves shipment of samples to diagnostic or collaborating laboratories.

Additional duties include, but are not limited to:

• Processing of woodchuck samples (blood, serum, liver, other tissues)
• Quantitative viral assays (woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV)), including slot blots for viral DNA, Southern and Northern hybridization for viral DNA and RNA, and real time PCR for viral DNA and RNA
• Host immune response assays, including proliferation assays for T-cells, ELISpot assays for IFN-gamma secreting T-cells, FACS-based degranulation assays for cytotoxic T lymphocytes, real time PCR for immune markers (cytokines and leukocyte surface markers) in whole blood lysates and liver biopsies
• ELISAs for viral proteins or antibodies against these proteins
• Organization of sample records and data from the above assays in standard spread sheets

Work Interactions

The Department of Microbiology and Immunology is dedicated to the study of infectious diseases caused by diverse pathogens, including basic research on and treatment of HBV in the woodchuck animal model. The individual is a team member of the PI’s group and critical for the sample processing and analysis from ongoing and future woodchuck studies. He/she reports directly to the PI. He/she interacts with other members of the PI’s group to coordinate sample processing and analysis. He/she interacts further with Undergraduate and Graduate students concerning sample processing and analysis.

Requirements and Qualifications

• Bachelor’s degree or higher with prior laboratory experience in general for several years (2-5 years). A preference will be given to individuals with prior experience with viral and host assays for HBV or other viruses
• Prior experience in performing sample processing and analysis under supervision and/or independently
• Must be willing and able to acquire skills and experience with woodchuck sample-specific assays
• Note that some of the above assays may require university certification for direct handling and conducting assays with radioisotopes (32P).
• Incumbent must be able to follow establish standardized protocols and procedures for routine use in the laboratory, as applied to woodchuck samples (i.e., most assays are lab-generated and cannot be purchased commercially)
• Must be able to perform new protocols and procedures like the above assays including applications of new reagents, and to use appropriate methodologies to validate results
• Must be able to conduct analysis to ensure correct and precise specificity of signals against appropriate standards and other controls
• The candidate is expected to ensure that high quality results are achieved in a timely and organized manner and that data obtained can meet the scientific objectives of the study.

Work Mode Designation

This position has been designated as On-Campus. Please note that work mode designations are regularly reviewed in order to meet the evolving needs of the University. Such review may necessitate a change to a position’s mode of work designation. Complete details about Georgetown University’s mode of work designations for staff positions can be found on the Department of Human Resources website: https://hr.georgetown.edu/mode-of-work-designation.

Pay Range:

The projectedsalary or hourly pay range for this position which represents the full range of anticipated compensation is:

$18.40 - $27.33

Compensation is determined by a number of factors including, but not limited to, the candidate’s individual qualifications, experience, education, skills, and certifications, as well as the University’s business needs and external factors.

EEO Statement:

GU is an Equal Opportunity Employer. All qualified applicants are encouraged to apply, and will receive consideration for employment without regard to age, citizenship, color, disability, family responsibilities, gender identity and expression, genetic information, marital status, matriculation, national origin, race, religion, personal appearance, political affiliation, sex, sexual orientation, veteran status, or any other characteristic protected by law.

Benefits:

Georgetown University offers a comprehensive and competitive benefit package that includes medical, dental, vision, disability and life insurance, retirement savings, tuition assistance, work-life balance benefits, employee discounts and an array of voluntary insurance options. You can learn more about benefits and eligibility on the Department of Human Resources website.