Research Assistant III

Georgetown University; United States; Presencial; USD 25.000 - 38.000; Jornada completa

Ventajas ofrecidas por este puesto de trabajo

Medical, dental, vision insurance

Tuition assistance

Work-life balance benefits

Descripción de la vacante

Georgetown University Medical Center, Department of Microbiology and Immunology, seeks a Research Assistant III (Laboratory Technician) to support grant-funded woodchuck studies and supervise students in sample processing and laboratory assays.

The role involves handling woodchuck samples, performing viral and immune assays (ELISA, qPCR, ELISpot, FACS), and maintaining organized data records for ongoing studies on HBV/WHV.

Formación

• Bachelor’s degree or higher with laboratory experience.
• Experience with viral and host assays for HBV or other viruses.
• Ability to perform sample processing and analysis with minimal supervision.
• Willing to acquire woodchuck-specific assays.
• Follow established protocols and ensure data quality.

Responsabilidades

• Process woodchuck samples (blood, serum, liver, other tissues).
• Perform viral assays for WHV and HBV, including blotting and RT-PCR.
• Conduct T-cell proliferation and ELISpot assays, and FACS-based degranulation.
• Perform RT-PCR for immune markers in blood and liver.
• Prepare ELISAs for viral proteins or antibodies.
• Organize sample records and data in spreadsheets.

Conocimientos

Laboratory experience

ELISA

qPCR

FACS

ELISpot

Cell proliferation

Data management

Training students

Educación

Bachelor’s degree or higher

Herramientas

Southern blot

Northern blot

Slot blot

Real-time PCR

Descripción del empleo

Job Overview

We are looking to hire a Research Assistant III (Laboratory Technician) within the Department of Microbiology and Immunology, Georgetown University Medical Center, School of Medicine – Georgetown University. This position is critical to the successful implementation of grant-funded studies in the woodchuck animal model, and as such, it is expected that the applicant has prior laboratory experience and is familiar with routine assays and methods, such as ELISA and quantitative PCR. Together with the PI, he/she coordinates and manages woodchuck-specific sample processing and then performs all assays/methods independently, including the determinations of viral titers and immune responses in blood, liver and other tissues of woodchucks. The individual is responsible for the handling and processing of stat and archived samples. He/she eventually trains and supervises Undergraduate and Graduate students during internships in relevant sample processing and laboratory assays. This position also involves shipment of samples to diagnostic or collaborating laboratories.

Additional duties include, but are not limited to:

• Processing of woodchuck samples (blood, serum, liver, other tissues)
• Quantitative viral assays (woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV)), including slot blots for viral DNA, Southern and Northern hybridization for viral DNA and RNA, and real time PCR for viral DNA and RNA
• Host immune response assays, including proliferation assays for T-cells, ELISpot assays for IFN-gamma secreting T-cells, FACS-based degranulation assays for cytotoxic T lymphocytes, real time PCR for immune markers (cytokines and leukocyte surface markers) in whole blood lysates and liver biopsies
• ELISAs for viral proteins or antibodies against these proteins
• Organization of sample records and data from the above assays in standard spread sheets

Work Interactions

The Department of Microbiology and Immunology is dedicated to the study of infectious diseases caused by diverse pathogens, including basic research on and treatment of HBV in the woodchuck animal model. The individual is a team member of the PI’s group and critical for the sample processing and analysis from ongoing and future woodchuck studies. He/she reports directly to the PI. He/she interacts with other members of the PI’s group to coordinate sample processing and analysis. He/she interacts further with Undergraduate and Graduate students concerning sample processing and analysis.

Requirements and Qualifications

• Bachelor’s degree or higher with prior laboratory experience in general for several years (2-5 years).
• A preference will be given to individuals with prior experience with viral and host assays for HBV or other viruses.
• Prior experience in performing sample processing and analysis under supervision and/or independently.
• Must be willing and able to acquire skills and experience with woodchuck sample-specific assays.
• Note that some of the above assays may require university certification for direct handling and conducting assays with radioisotopes (32P).
• Incumbent must be able to follow established standardized protocols and procedures for routine use in the laboratory, as applied to woodchuck samples (i.e., most assays are lab-generated and cannot be purchased commercially).
• Must be able to perform new protocols and procedures like the above assays including applications of new reagents, and to use appropriate methodologies to validate results.
• Must be able to conduct analysis to ensure correct and precise specificity of signals against appropriate standards and other controls.
• The candidate is expected to ensure that high quality results are achieved in a timely and organized manner and that data obtained can meet the scientific objectives of the study.

Work Mode Designation

This position has been designated as On-Campus. Please note that work mode designations are regularly reviewed in order to meet the evolving needs of the University. Such review may necessitate a change to a position’s mode of work designation. Complete details about Georgetown University’s mode of work designations for staff positions can be found on the Department of Human Resources website: https://hr.georgetown.edu/mode-of-work-designation.

Pay Range

Pay Range: The projected salary or hourly pay range for this position which represents the full range of anticipated compensation is: $18.40 - $27.33. Compensation is determined by a number of factors including, but not limited to, the candidate’s individual qualifications, experience, education, skills, and certifications, as well as the University’s business needs and external factors.

EEO Statement

GU is an Equal Opportunity Employer. All qualified applicants are encouraged to apply, and will receive consideration for employment without regard to age, citizenship, color, disability, family responsibilities, gender identity and expression, genetic information, marital status, matriculation, national origin, race, religion, personal appearance, political affiliation, sex, sexual orientation, veteran status, or any other characteristic protected by law.

Benefits

• Georgetown University offers a comprehensive and competitive benefit package that includes medical, dental, vision, disability and life insurance, retirement savings, tuition assistance, work-life balance benefits, employee discounts and an array of voluntary insurance options.
• Georgetown University offers a comprehensive and competitive benefit package that includes medical, dental, vision, disability and life insurance, retirement savings, tuition assistance, work-life balance benefits, employee discounts and an array of voluntary insurance options.

Company Culture

We’re a leading research university with a heart. Founded in the decade that the U.S. Constitution was signed, we’re the nation’s oldest Catholic and Jesuit university. Today we’re the forward-looking, diverse community devoted to social justice, restless inquiry and respect for each person’s individual needs and talents. Grounded in our Jesuit, Catholic identity, the Spirit of Georgetown undergirds everything we do. Watch this video to learn more. Learn how HR CARES for faculty and staff, nurturing an environment of mutual respect where imaginations flourish and ideas are realized to positively impact the world.

You can learn more about benefits and eligibility on the Department of Human Resources website.