Protein Sciences & Structural Biology Scientist I

Broad Institute of MIT and Harvard; Cambridge (MA); On-site; USD 88,000 - 124,000; Full time

Benefits offered by this job

Medical insurance

Dental insurance

Vision insurance

401(k)

Job summary

The Broad Institute in Cambridge, MA invites a talented scientist to join the Center for the Development of Therapeutics (CDoT) as a protein biochemistry specialist. You will design and execute strategies to clone and express recombinant proteins, purify them, and support protein crystallography projects within cross‑functional teams.

Applicants should have a Ph.D. in Biophysics, Biochemistry, Molecular Biology, Structural Biology or related field and 0-2+ years of industrial or academic

Qualifications

• Ph.D. in Biophysics, Biochemistry, Molecular Biology, Structural Biology, or related field.
• Experience with recombinant protein purification using chromatography.
• Cloning and expressing proteins in multiple expression systems.
• Experience with protein characterization and biophysical binding studies.
• Strong collaboration in cross-functional teams.

Responsibilities

• Developing and executing strategies to clone and express recombinant proteins in a variety of expression systems.
• Purifying tagged or native proteins using standard purification (FPLC systems and column chromatography) and analytical techniques (SDS‑PAGE, Western blot, light scattering, and protein LC/MS).
• With training, produce high quality protein crystal structures and co‑crystal structures, carrying out crystallization screening through to structure refinement.
• Initiating and completing routine protein construct design and production.
• Demonstrating the ability to interpret experimental outcomes and proposing follow‑up studies.
• Troubleshooting and solving scientific problems related to protein production independently.
• Operating and maintaining lab equipment independently, including protein purification systems like an AKTA pure.
• Investigating new technologies related to protein expression and purification.
• Documenting work and effectively presenting results in cross‑functional project teams.

Education

Ph.D in Biophysics, Biochemistry, Molecular Biology, Structural Biology or related life science field

Tools

AKTA Pure

Mass spectrometry

Job description

The Broad Institute in Cambridge, MA invites a talented scientist to join the Center for the Development of Therapeutics (CDoT) as a protein biochemistry specialist. You will design and execute strategies to clone and express recombinant proteins, purify them, and support protein crystallography projects within cross‑functional teams.

Applicants should have a Ph.D. in Biophysics, Biochemistry, Molecular Biology, Structural Biology or related field and 0-2+ years of industrial or academic