Protein Production & Purification Associate

Flagship Pioneering Co-Op Program; Cambridge (MA); On-site; USD 65,000 - 94,000; Full time

Job summary

Flagship Pioneering seeks a Research Associate II to expand protein production across bulk expression and high-throughput panels. You’ll execute scalable expression, purification, normalization, and characterization to support design-build-test cycles.

Collaboration with engineering and assay teams, careful record-keeping, and adaptability to fast timelines are essential. The role emphasizes hands-on recombinant protein work in a dynamic, matrixed environment with attention to traceability and

Qualifications

• Hands-on recombinant protein expression and purification workflows.
• Experience with multi-liter culture growth and harvest.
• Proficiency with multiple purification modalities.

Responsibilities

• Execute bulk expression campaigns from transformation to lysis and harvest.
• Design and run preparative purification on ÄKTA FPLC systems (UNICORN).
• Perform high-throughput expression and purification in plate formats.

Skills

Protein expression

Purification

Analytical skills

Record-keeping

Team collaboration

Education

Bachelor’s degree in biochemistry or related field

Tools

ÄKTA FPLC

UNICORN

KingFisher

Liquid handling platforms

Job description

Flagship Pioneering seeks a Research Associate II to expand protein production across bulk expression and high-throughput panels. You’ll execute scalable expression, purification, normalization, and characterization to support design-build-test cycles.

Collaboration with engineering and assay teams, careful record-keeping, and adaptability to fast timelines are essential. The role emphasizes hands-on recombinant protein work in a dynamic, matrixed environment with attention to traceability and